Insights into the molecular basis of gastric mucosa as a first step for using Raman microscopy in paediatrics

Jiří Bufka; Lenka Vaňková; Josef Sýkora; Magdaléna Daumová; Petr Bouř; Jan Schwarz

doi:10.1016/j.heliyon.2024.e36231

Insights into the molecular basis of gastric mucosa as a first step for using Raman microscopy in paediatrics

Heliyon. 2024 Aug 14;10(16):e36231. doi: 10.1016/j.heliyon.2024.e36231. eCollection 2024 Aug 30.

Authors

Jiří Bufka^{1

2}, Lenka Vaňková³, Josef Sýkora¹, Magdaléna Daumová⁴, Petr Bouř², Jan Schwarz¹

Affiliations

¹ Department of Paediatrics, Faculty of Medicine in Pilsen, Faculty Hospital, Charles University in Prague, Pilsen, Czech Republic.
² Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences Prague, Czech Republic.
³ Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Czech Republic.
⁴ Sikl's Department of Pathology, Faculty of Medicine in Pilsen, Charles University, Czech Republic.

Abstract

Recent advances in endoscopic technology have allowed detailed observation of the gastric mucosa, including Raman microscopy and spectroscopy. To explore the possibilities for future diagnostic use, we discuss the measurements and molecular markers found in this tissue. The Raman spectra of 16 samples of antral mucosa and 16 samples of corpus gastric mucosa obtained from healthy donors were analysed. A stable protocol for measuring reproducible spectra was established. These data suggest that many biomarkers can be used for the rapid analysis of metabolic states and future investigations into the pathogenesis of gastrointestinal diseases.

Keywords: Diagnostic methods; Gaster; Measurement protocol; Mucosa; Paediatrics; Raman spectroscopy.