The Interactions of Resveratrol and Sodium Valproate on Penicillin-Induced Epilepsy Model: Electrophysiological and Molecular Study

Zeynep Kasap Acungil; Secil Erden Tayhan; Nazan Goksen Tosun; Tuncer Nacar

doi:10.1007/s12035-024-04502-z

The Interactions of Resveratrol and Sodium Valproate on Penicillin-Induced Epilepsy Model: Electrophysiological and Molecular Study

Mol Neurobiol. 2024 Sep 24. doi: 10.1007/s12035-024-04502-z. Online ahead of print.

Authors

Zeynep Kasap Acungil¹, Secil Erden Tayhan², Nazan Goksen Tosun³, Tuncer Nacar⁴

Affiliations

¹ Department of Physiotherapy and Rehabilitation, Faculty of Health Science, Tokat Gaziosmanpasa University, Tokat, Turkey. zeynep.kasap@gop.edu.tr.
² Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tokat Gaziosmanpasa University, Tokat, Turkey.
³ Department of Medical Services and Techniques, Vocational School of Health Services, Tokat Gaziosmanpasa University, Tokat, Turkey.
⁴ Department of Physiology, Faculty of Medicine, Yüksek Ihtisas University, Ankara, Turkey.

PMID: 39316354
DOI: 10.1007/s12035-024-04502-z

Abstract

Epilepsy represents the most prevalent chronic neurological disease, characterized by spontaneous recurrent seizures. In experimental epilepsy models created by different methods, resveratrol has been demonstrated to reduce epileptiform activity and exhibit neuroprotective properties. A penicillin-induced model of epileptogenesis was used to investigate the effects of resveratrol and its combination with sodium valproate on epileptiform activity. The study design was an in vivo animal experimental study. Forty Wistar-albino rats were divided into five groups, each with eight rats. The groups are categorized as the saline group, penicillin group (only penicillin), resveratrol group, sodium valproate group, and resveratrol + sodium valproate group. ECoG recording was taken for 180 min in all groups and statistically evaluated. GABAα1, mGluR1/mGluR5, NMDAR1 receptor expressions in the hippocampus, and S100B level in serum were measured. The spike frequency decreased statistically to 60th min in the sodium valproate group and 150th min in the resveratrol group. The spike frequency decreased statistically in the 20th min and later measurements of the recording in the resveratrol + sodium valproate group. GABAα1 receptor expression was increased in all groups compared to the penicillin group. mGluR1/mGluR5, NMDAR1 receptor expression was decreased in all groups compared to the penicillin group. Serum S100B level increased in all groups compared to the penicillin group. There was no statistically significant difference in epileptiform activity when resveratrol alone was administered in the penicillin-induced epilepsy model. Resveratrol co-administered with sodium valproate significantly reduced epileptiform activity. Co-administration of the sodium valproate + resveratrol group made the receptor level's highest GABAα1receptor expression at receptors.

Keywords: GABA; Glutamate; Penicillin epilepsy model; Resveratrol; S100B.