Using ApE for In Silico Golden Gate Cloning

M Wayne Davis; Erik M Jorgensen

doi:10.1007/978-1-0716-4220-7_5

Using ApE for In Silico Golden Gate Cloning

Methods Mol Biol. 2025:2850:79-87. doi: 10.1007/978-1-0716-4220-7_5.

Authors

M Wayne Davis¹, Erik M Jorgensen²

Affiliations

¹ School of Biological Sciences, HHMI, University of Utah, Salt Lake City, UT, USA. wdavis@biology.utah.edu.
² School of Biological Sciences, HHMI, University of Utah, Salt Lake City, UT, USA.

PMID: 39363067
DOI: 10.1007/978-1-0716-4220-7_5

Abstract

Golden Gate cloning allows rapid and reliable assembly of multiple DNA fragments in a defined orientation. Golden Gate cloning requires careful design of the restriction fragment overhangs to minimize undesired products and to generate the desired junctions. The ApE (A plasmid Editor) software package can assist in silico design of input fragments or to generate expected assembly products.

Keywords: ApE; Golden Gate assembly; Golden Gate design; In silico cloning.

MeSH terms

Cloning, Molecular* / methods
Computational Biology / methods
Computer Simulation
Plasmids / genetics
Software*