The aim of this study was to increase the bioconversion efficiency (lipid accumulation) of black soldier fly larvae while simultaneously increasing biodiesel production through a feed-shifting strategy. Feeding with low-lipid feed promoted an increase in larval weight, while high-lipid feed resulted in greater lipid accumulation. Based on this result, a feed-shifting strategy was introduced, which consisted of two stages: first, increasing larval body weight using low-lipid feed, followed by lipid induction for biodiesel production using high-lipid feed. The use of this strategy resulted in an increase in the dry weight of larvae by ≥16 % compared to single feeding systems. This led to a 20 % increase in biodiesel productivity. The waste reduction ratio was enhanced due to the higher bioconversion rate in the feed-shifting strategy compared to that in the single feeding systems. The feed-shifting strategy would contribute to the enhancement of waste-to-energy efficiency.
Keywords: Direct conversion; Lipid induction; Sustainable food waste management; Two-stage cultivation; Waste-to-energy.
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