Actin-mediated avoidance of tricellular junction influences global topology at the Arabidopsis shoot apical meristem

Abstract

Division plane orientation contributes to cell shape and topological organization, playing a key role in morphogenesis, but the precise physical and molecular mechanism influencing these processes remains largely obscure in plants. In particular, it is less clear how the placement of the new walls occurs in relation to the walls of neighboring cells. Here, we show that genetic perturbation of the actin cytoskeleton results in more rectangular cell shapes and higher incidences of four-way junctions, perturbing the global topology of cells in the shoot apical meristem of Arabidopsis thaliana. Actin mutants also exhibit changes in the expansion rate of the new versus the maternal cell wall after division, affecting the evolution of internal angles at tricellular junctions. Further, the increased width of the preprophase band in the actin mutant contributes to inaccuracy in the placement of the new cell wall. Computational simulation further substantiates this hypothesis and reproduces the observed cell shape defects.

Keywords: Arabidopsis; CP: Cell biology; CP: Plants; actin; cell division; cell packing; four-way junction; microtubules; shoot apical meristem; topology.