Sensitive Hydraulic and Stomatal Decline in Extreme Drought Tolerant Species of California Ceanothus

Joseph Zailaa; Santiago Trueba; Marvin Browne; Leila R Fletcher; Thomas N Buckley; Craig R Brodersen; Christine Scoffoni; Lawren Sack

doi:10.1111/pce.15208

Sensitive Hydraulic and Stomatal Decline in Extreme Drought Tolerant Species of California Ceanothus

Plant Cell Environ. 2024 Oct 27. doi: 10.1111/pce.15208. Online ahead of print.

Authors

Joseph Zailaa^{1

2

3}, Santiago Trueba^{3

4}, Marvin Browne^{3

5}, Leila R Fletcher^{1

3}, Thomas N Buckley⁶, Craig R Brodersen¹, Christine Scoffoni², Lawren Sack³

Affiliations

¹ School of the Environment, Yale University, New Haven, Connecticut, USA.
² Department of Biological Sciences, California State University, Los Angeles, Los Angeles, California, USA.
³ Department of Ecology and Evolutionary Biology, University of California, Los Angeles, Los Angeles, California, USA.
⁴ AMAP, University of Montpellier, CIRAD, CNRS, INRAE, IRD, Montpellier, France.
⁵ Department of Earth System Science, Stanford University, Stanford, CA, USA.
⁶ Department of Plant Sciences, University of California, Davis, Davis, California.

PMID: 39462892
DOI: 10.1111/pce.15208

Abstract

Identifying the physiological mechanisms by which plants are adapted to drought is critical to predict species responses to climate change. We measured the responses of leaf hydraulic and stomatal conductances (K_leaf and g_s, respectively) to dehydration, and their association with anatomy, in seven species of California Ceanothus grown in a common garden, including some of the most drought-tolerant species in the semi-arid flora. We tested for matching of maximum hydraulic supply and demand and quantified the role of decline of K_leaf in driving stomatal closure. Across Ceanothus species, maximum K_leaf and g_s were negatively correlated, and both K_leaf and g_s showed steep declines with decreasing leaf water potential (i.e., a high sensitivity to dehydration). The leaf water potential at 50% decline in g_s was linked with a low ratio of maximum hydraulic supply to demand (i.e., maximum K_leaf:g_s). This sensitivity of g_s, combined with low minimum epidermal conductance and water storage, could contribute to prolonged leaf survival under drought. The specialized anatomy of subg. Cerastes includes trichomous stomatal crypts and pronounced hypodermis, and was associated with higher water use efficiency and water storage. Combining our data with comparative literature of other California species, species of subg. Cerastes show traits associated with greater drought tolerance and reliance on leaf water storage relative to other California species. In addition to drought resistance mechanisms such as mechanical protection and resistance to embolism, drought avoidance mechanisms such as sensitive stomatal closure could contribute importantly to drought tolerance in dry-climate adapted species.

Keywords: California floristic province; Rhamnaceae; leaf hydraulics; physiological thresholds; stress response; trait‐based ecology.

Grants and funding

This work was supported by National Science Foundation grants 2017949, 1951244, 1943583, 1557906 and 2307341, and USDA NIFA Hatch project 1016439. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.