Establishment of Patient-Derived Organoids from Colorectal Cancer Resection Samples

Didem Seven; Szilvia Baron; Henner F Farin

doi:10.1007/7651_2024_575

Establishment of Patient-Derived Organoids from Colorectal Cancer Resection Samples

Methods Mol Biol. 2024 Nov 10. doi: 10.1007/7651_2024_575. Online ahead of print.

Authors

Didem Seven¹, Szilvia Baron^{2

3}, Henner F Farin^{2

3}

Affiliations

¹ Yeditepe University School of Medicine, Medical Genetics Department, Istanbul, Turkey. didem.seven@yeditepe.edu.tr.
² Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt am Main, Germany.
³ Frankfurt Cancer Institute, Goethe University, Frankfurt am Main, Germany.

PMID: 39520596
DOI: 10.1007/7651_2024_575

Abstract

Colorectal cancer (CRC) organoids can serve as powerful preclinical cell models that accurately reflect individual tumor characteristics. Establishing a patient-derived CRC biobank facilitates a wide range of applications, including basic oncology research, new drug discovery, drug testing, and personalized medicine. This chapter details the process of generation of organoids from surgical samples of primary and metastatic CRC as well as from tumor adjacent normal colon tissues. Furthermore, best practices for cultivation and cryostorage of CRC organoids are described.

Keywords: Biobank; Colon organoids; Colorectal cancer; Patient-derived organoids; Stem cells.