Determination of the biological activities of endemic Allium lazikkiyense and its phytochemical profile by LC-MS/MS analysis

Food Chem. 2025 Feb 1;464(Pt 3):141930. doi: 10.1016/j.foodchem.2024.141930. Epub 2024 Nov 4.

Abstract

This study investigated the phytochemical contents and in vitro antioxidant, enzyme inhibitory and anticancer properties of ethanol, methanol, and dichloromethane extracts prepared from aerial parts and onion peels of endemic Allium lazikkiyense Koçyiğit, Özhatay & E.Kaya. Ethanol extracts showed the highest antioxidant activity among the all extracts in terms of total phenolic and flavonoid contents as well as DPPH scavenging and iron reducing potentials. LC-MS/MS analysis results showed that aerial parts rich in fumaric acid, p-coumaric acid, ascorbic acid and hyperoside while onion peels rich in sarsasapogenin, caffeic acid and fumaric acid. Dichloromethane extracts showed a strong inhibitory effect on α-glucosidase, while all extracts exhibited low inhibitory activity on α-amylase, acetylcholinesterase and butyrylcholinesterase. Also, extracts from onion peels exhibited potent cytotoxic effect against MCF7 human breast cancer cells. The present study revealed that A. lazikkiyense could be a good natural source for antioxidant, α-glucosidase inhibitory, and anticancer activities.

Keywords: Allium lazikkiyense; Anticancer; Antioxidant; Enzyme inhibitory.

MeSH terms

  • Acetylcholinesterase / metabolism
  • Allium* / chemistry
  • Antioxidants* / chemistry
  • Antioxidants* / pharmacology
  • Butyrylcholinesterase / metabolism
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology
  • Humans
  • Liquid Chromatography-Mass Spectrometry
  • MCF-7 Cells
  • Onions / chemistry
  • Phytochemicals* / chemistry
  • Phytochemicals* / pharmacology
  • Plant Extracts* / chemistry
  • Plant Extracts* / pharmacology
  • Tandem Mass Spectrometry*
  • alpha-Amylases / antagonists & inhibitors
  • alpha-Glucosidases / metabolism

Substances

  • Plant Extracts
  • Phytochemicals
  • Antioxidants
  • alpha-Amylases
  • alpha-Glucosidases
  • Enzyme Inhibitors
  • Acetylcholinesterase
  • Butyrylcholinesterase