Reconstruction of the Reaction of Andalusicin Lantibiotic Modification by Lanthionine Synthetase AncKC in a Heterologous Escherichia coli System

N Z Mirzoeva; S O Pipiya; Yu A Mokrushina; M V Serebryakova; A A Grigoreva; S A Dubiley; S S Terekhov; I V Smirnov

doi:10.32607/actanaturae.27347

Reconstruction of the Reaction of Andalusicin Lantibiotic Modification by Lanthionine Synthetase AncKC in a Heterologous Escherichia coli System

Acta Naturae. 2024 Jul-Sep;16(3):46-50. doi: 10.32607/actanaturae.27347.

Authors

N Z Mirzoeva¹, S O Pipiya¹, Yu A Mokrushina^{1

2}, M V Serebryakova³, A A Grigoreva^{4

5}, S A Dubiley^{4

5}, S S Terekhov¹, I V Smirnov^{1

2}

Affiliations

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russian Federation.
² Department of Chemistry, Lomonosov Moscow State University, Moscow, 119991 Russian Federation.
³ A.N. Belozersky Institute of Physicochemical Biology, Moscow, 119991 Russian Federation.
⁴ Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, 121205 Russian Federation.
⁵ Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russian Federation.

Abstract

The increasing resistance of microorganisms to antibiotics makes it a necessity that we search for new antimicrobial agents. Due to their genetically encoded nature, peptides are promising candidates for new antimicrobial drugs. Lantipeptide andalusicin exhibits significant antimicrobial activity against Gram-positive bacteria, making it a promising scaffold for the development of DNA-encoded libraries of lantibiotics. In this study, the modification reaction of andalusicin by class III lanthionine synthetase AncKC was reconstructed in a heterologous Escherichia coli system. The results obtained open possibilities for creating novel peptide- based antimicrobial agents.

Keywords: antimicrobial peptides; lanthipeptides; posttranslational modifications.