Bioactive functional materials have been focused recently because of their superior properties due to structural orientations. Herein, carbon quantum dots (CQDs) encapsulated chemically modified carboxymethyl cellulose (CMC) nanocomposites are designed for non-enzymatic ex vivo glucose sensing by a low-cost green technique. The N,N'-Dicyclohexylcarbodiimide (DCC) assisted Steglich esterification between CMC and glucose is responsible for the fluorescence "Turn ON-OFF" mechanism behind glucose detection. The in vitro sensing study derived a limit of detection (LOD) of 7 nM for glucose covering two linear ranges from 0 to 0.06 mM (R2 = 0.9346) and 1.28 to 61.44 mM (R2 = 0.9704). The sensing system maintained excellent stability under extreme ionic strength and pH conditions and the high selectivity is successfully studied against interferents such as homologous sugars, amino acids, cations, and anions. Clinical potential of the proposed sensor is validated through direct testing in human blood samples against commercial glucometer. The sensor proved its reliability with recovery rates of 100 ± 5 % confirming its promising application in glucose monitoring towards effective diabetes management.
Keywords: CQD; Carboxymethyl cellulose; Glucose sensing; Human blood serum; Turn ON-OFF mechanism.
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