Adsorption and release pattern of recombinant human bone morphogenic proatin 2 onto different bone grafts and its consequent osteoblasts` activation and neutrophils` priming

Adir Cohen; Tom Avraham Verkauf; Nardy Casap; Tali Chackartchi; David Polak

doi:10.1016/j.archoralbio.2024.106123

Adsorption and release pattern of recombinant human bone morphogenic proatin 2 onto different bone grafts and its consequent osteoblasts` activation and neutrophils` priming

Arch Oral Biol. 2024 Oct 30:170:106123. doi: 10.1016/j.archoralbio.2024.106123. Online ahead of print.

Authors

Adir Cohen¹, Tom Avraham Verkauf², Nardy Casap¹, Tali Chackartchi², David Polak³

Affiliations

¹ Department of Oral and Maxillofacial Surgery, Hadassah Medical Center, Faculty, of Dental Medicine, Hebrew University of Jerusalem, Israel.
² Department of Periodontology, Hadassah Medical Center, Faculty, of Dental Medicine, Hebrew University of Jerusalem, Israel.
³ Department of Periodontology, Hadassah Medical Center, Faculty, of Dental Medicine, Hebrew University of Jerusalem, Israel. Electronic address: polak@mail.huji.ac.il.

PMID: 39561521
DOI: 10.1016/j.archoralbio.2024.106123

Abstract

Objectives: Controlled long-term delivery of recombinant human bone morphogenic proatin 2 (rhBMP2) eluted in a collagen scaffolds suffer from only a high initial burst release. The purpose of the current study was to investigate the long-term delivery of rhBMP2 when mixed with different bone grafts and its impact on osteoblastic activity and neutrophil priming.

Methods: rhBMP2 was separately mixed with xenograft, allograft, or alloplast and incubated for 30 days. Levels of BMP2 adsorption and their release were measured using immunofluorescence and ELISA respectively. The supernatants from the grafts were then incubated with either osteoblast (Saos-2 cells) or neutrophils (differentiated from HL60) for alkaline phosphatase and oxidative stress measurements respectively. Gene expression of osteoblast functionality and neutrophil priming were measured with qRT-PCR.

Results: rhBMP2 was adsorbed onto all tested grafts, with a superior effect of alloplast. The release of the rhBMP2 from all grafts was similar and sustained for 30 days with the lowest levels in the alloplast group. Activation of osteoblast was robust in the allograft and xenograft groups, concomitant with elevated osteocalcin expression. Neutrophil priming was greatest in the xenograft group, together with elevated expression of interleukin 1β.

Conclusion: rhBMP2 with bone graft material constitutes its sustained release over time. This, in turn, robust osteoblast and neutrophil activity.

Keywords: Bone grafts; Neutrophils; Osteoblasts; Recombinant human bone morphogenic proatin 2.