Marburg Virus Minigenome Assays

Ellen L Suder; Whitney A Scoon; Elke Mühlberger

doi:10.1007/978-1-0716-4256-6_9

Marburg Virus Minigenome Assays

Methods Mol Biol. 2025:2877:129-139. doi: 10.1007/978-1-0716-4256-6_9.

Authors

Ellen L Suder¹, Whitney A Scoon¹, Elke Mühlberger²

Affiliations

¹ Department of Virology, Immunology and Microbiology, Chobanian & Avedisian School of Medicine and National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
² Department of Virology, Immunology and Microbiology, Chobanian & Avedisian School of Medicine and National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA. muehlber@bu.edu.

PMID: 39585618
DOI: 10.1007/978-1-0716-4256-6_9

Abstract

This chapter describes minigenome systems for Marburg virus (MARV), which reconstitute the viral polymerase complex functions of gene expression and genome replication. Procedures covered herein include passage and seeding of cells, transfection, sample collection, and reporter gene assays.

Keywords: Filoviruses; Genome replication and transcription; Marburg virus; Minigenome system; Nonsegmented negative-sense RNA virus; Reporter gene assay.

MeSH terms

Animals
Cell Line
Genes, Reporter
Genome, Viral*
Humans
Marburgvirus* / genetics
Transfection / methods
Virus Replication / genetics