Effect of CD10-positive cells on osteogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells

Tomoaki Sakurai; Masakazu Ishii; Haruka Miyata; Nao Ikeda; Fumio Suehiro; Naohiro Komabashiri; Yurika Oura; Masahiro Nishimura

doi:10.1016/j.archoralbio.2024.106135

Effect of CD10-positive cells on osteogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells

Arch Oral Biol. 2024 Nov 23:170:106135. doi: 10.1016/j.archoralbio.2024.106135. Online ahead of print.

Authors

Tomoaki Sakurai¹, Masakazu Ishii², Haruka Miyata¹, Nao Ikeda¹, Fumio Suehiro¹, Naohiro Komabashiri¹, Yurika Oura¹, Masahiro Nishimura¹

Affiliations

¹ Department of Oral and Maxillofacial Prosthodontics, Kagoshima University Graduate school of Medical and Dental Science, Kagoshima 890-8544, Japan.
² Department of Oral and Maxillofacial Prosthodontics, Kagoshima University Graduate school of Medical and Dental Science, Kagoshima 890-8544, Japan. Electronic address: masai@dent.kagoshima-u.ac.jp.

PMID: 39591929
DOI: 10.1016/j.archoralbio.2024.106135

Abstract

Objective: This study was aimed at investigating the effect of CD10-positive cells within the maxillary/mandibular bone marrow-derived mesenchymal stem cells (MBMSCs) on osteogenic differentiation of MBMSCs.

Design: CD10 expression in iliac bone marrow-derived MSCs (IBMSCs), MBMSCs, and gingival fibroblasts was measured using flow cytometry. The osteogenic potential of 19 MBMSC lines was evaluated, and based on it, they were classified into osteogenic-High and osteogenic-Low groups. The percentage of CD10-positive cells in each group was compared. Effect of coculturing gingival fibroblasts and CD10-positive cells on the osteogenic potential of MBMSCs was also assessed. Expression of tissue inhibitor of metalloprotease-1 (TIMP-1) in osteogenic-High and osteogenic-Low MBMSCs was measured using quantitative real-time polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assay. The molecular mechanisms underlying the regulation of osteogenic differentiation in MBMSCs were investigated.

Results: CD10 was not expressed in IBMSCs, but was highly expressed in fibroblasts. In MBMSCs, the CD10-positivity rate varied considerably between cells. MBMSCs with a high-CD10 positivity rate showed low osteogenic potential. Coculture with fibroblasts or CD10-positive cells reduced the osteogenic potential of MBMSCs. TIMP-1 was highly expressed in CD10-positive cells, and osteogenic-Low MBMSCs showed significantly higher TIMP-1 expression compared with osteogenic-High MBMSCs. β-catenin signaling was suppressed in osteogenic-Low MBMSCs.

Conclusion: This study revealed that TIMP-1 secreted from CD10-positive cells may be involved in the suppression of the osteogenic potential of MBMSCs by contamination with CD10-positive cells. This finding provides important insights for developing bone regeneration therapies using MBMSCs.

Keywords: CD10; Maxillary/mandibular bone; Mesenchymal stem cells; Osteogenic differentiation; TIMP-1; β-Catenin.