Evaluation of diagnostic performances of Pro-neurotensin and Heart-type fatty acid binding protein as reliable biomarkers for cardiovascular diseases

J Immunoassay Immunochem. 2025 Jan 2;46(1):49-74. doi: 10.1080/15321819.2024.2430332. Epub 2024 Nov 26.

Abstract

Aim: In 2019, cardiovascular diseases accounted for 32% of global deaths. So, early detection of cardiac disorders is crucial. The study aimed to examine the suitability of Pro-neurotensin and Heart-type fatty acid binding protein as dependable biomarkers for cardiac patients with Heart failure as a primary diagnosis.

Methodology: The prospective study involved 204 Egyptian volunteers (100 cardiac patients and 104 controls) enrolled from El-Sahel Teaching Hospital in Cairo, Egypt, between October 2022 and May 2023. Inclusion criteria included a high risk of cardiovascular events with symptoms like a fast or irregular pulse, shortness of breath, and fatigue. Exclusion criteria included asymptomatic individuals, cognitive disorders, and psychiatric conditions. The Research Ethics Committee approved the protocol. The consultant conducted a clinical examination of all patients and assessed their heart state. Serum ProNT and H-FABP were detected using a kit for the sandwich ELISA technique.

Results: ProNT and H-FABP were significantly elevated in patients compared to controls with p < 0.001. Demonstrated sensitivity of 81% and 84%, with a specificity of 89% and 91%, respectively.

Conclusion: Elevated ProNT and H-FABP levels are associated with severe CVDs, suggesting their potential as diagnostic biomarkers for patients, specifically those with heart failure, as a primary characteristic.

Keywords: Cardiac blood markers; Cardiomyopathy; FABP3; Heart failure; Neurotensin.

MeSH terms

  • Adult
  • Aged
  • Biomarkers* / blood
  • Cardiovascular Diseases* / blood
  • Cardiovascular Diseases* / diagnosis
  • Enzyme-Linked Immunosorbent Assay
  • Fatty Acid Binding Protein 3 / blood
  • Fatty Acid-Binding Proteins / blood
  • Female
  • Heart Failure / blood
  • Heart Failure / diagnosis
  • Humans
  • Male
  • Middle Aged
  • Natriuretic Peptide, Brain / blood
  • Prospective Studies

Substances

  • Biomarkers
  • Fatty Acid Binding Protein 3
  • Fatty Acid-Binding Proteins
  • FABP3 protein, human
  • Natriuretic Peptide, Brain