Soluble P-selectin as an inflammatory mediator potentially influencing endothelial activation in people living with HIV in sub-rural areas of Limpopo, South Africa

PLoS One. 2024 Nov 27;19(11):e0310056. doi: 10.1371/journal.pone.0310056. eCollection 2024.

Abstract

Objectives: There is a growing need to understand the potential role of soluble platelet selectin (sP-selectin) in sustained endothelial activation through increased levels of soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular adhesion-1 (sVCAM-1) in people living with HIV (PLWH) on highly active antiretroviral therapy (HAART).

Methodology: This was a cross-sectional study involving PLWH on HAART (n = 55), in comparison to PLWH not on treatment (HAART-naïve) (n = 29), and (iii) HIV negative controls (n = 48) from the Mankweng area in the Limpopo province, South Africa. We quantified serum levels of sP-selectin, together with sICAM-1 and sVCAM-1. Most of the HAART-exposed group were on treatment for <5 years. We further performed frequency distribution and descriptive statistics for categorical variables.

Results: Soluble P-selectin was positively correlated with sVCAM-1 (r = 0.469; p<0.001) in PLWH on HAART, even after adjusting for confounding factor such as age, BMI, and total cholesterol (r = 0.467; p<0.001). Moreover, in PLWH on HAART sP-selecting was independently associated with the release of sVCAM-1 (β = 0.445; p<0.001), even after adjusting for confounders (β = 0.475; p = 0.001). Serum levels of low-density lipoprotein cholesterol (LDL-C) (p = 0.004) and total cholesterol (p<0.001) were significantly higher in PLWH on HAART as compared to the HAART-naïve group.

Conclusion: There is a need for more studies to investigate the role of sP-selectin in promoting endothelial activation and CVD-risk in PLWH on HAART, especially within the sub-Saharan Africa region.

MeSH terms

  • Adult
  • Antiretroviral Therapy, Highly Active*
  • Cross-Sectional Studies
  • Endothelium, Vascular / metabolism
  • Female
  • HIV Infections* / blood
  • HIV Infections* / drug therapy
  • Humans
  • Inflammation Mediators / blood
  • Intercellular Adhesion Molecule-1 / blood
  • Male
  • Middle Aged
  • P-Selectin* / blood
  • Rural Population
  • South Africa / epidemiology
  • Vascular Cell Adhesion Molecule-1* / blood

Substances

  • P-Selectin
  • Vascular Cell Adhesion Molecule-1
  • Intercellular Adhesion Molecule-1
  • Inflammation Mediators

Grants and funding

Sidney Hanser received funding from the National Research Foundation (NRF) (Grant number: TTK2204082828). Phiwayinkosi V. Dludla also received funding from NRF (Grant numbers: 117829 and 141929). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.