Volatilome Study of Jabuticaba (Plinia cauliflora) Using a Hydrophilic Microporous Cartridge with Direct Immersion Solid Phase Microextraction and Gas Chromatography System With a Hybrid Quadrupole Time-Of-Flight Mass Spectrometry

Jhonatan Bispo de Oliveira; Samantha Christina Rodrigues; Helvécio Costa Menezes; Patterson Patrício de Souza; Zenilda de Lourdes Cardeal

doi:10.1002/jssc.70038

Volatilome Study of Jabuticaba (Plinia cauliflora) Using a Hydrophilic Microporous Cartridge with Direct Immersion Solid Phase Microextraction and Gas Chromatography System With a Hybrid Quadrupole Time-Of-Flight Mass Spectrometry

J Sep Sci. 2024 Dec;47(23):e70038. doi: 10.1002/jssc.70038.

Authors

Jhonatan Bispo de Oliveira¹, Samantha Christina Rodrigues¹, Helvécio Costa Menezes¹, Patterson Patrício de Souza², Zenilda de Lourdes Cardeal¹

Affiliations

¹ Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
² Departamento de Química, Centro Federal de Educação Tecnológica de Minas Gerais, Belo Horizonte, Brazil.

PMID: 39623530
DOI: 10.1002/jssc.70038

Abstract

Jabuticaba (Plinia cauliflora) is a typical subtropical Brazilian fruit with unique organoleptic properties and a high nutritional value. This study shows a qualitative analysis of jabuticaba peels with volatile and semi-volatile components harvested from Minas Gerais, Brazil. A new device, the hydrophilic microporous cartridge, was developed to extract jabuticaba peels' volatile/semi-volatile components using a solid-phase microextraction method by direct immersion. This cartridge protected the polymer phase fiber, preventing its breakage and impregnation of the material. The developed method is simple, using a few steps to prepare jabuticaba peel samples. The fiber selected for analysis was a divinylbenzene/carboxene/polydimethylsiloxane of 30/50 µm. A comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry system was used to analyze natural products in jabuticaba peels. The method was optimized by a factorial design and could detect 213 organic compounds. Of particular note is the detection of terpenes (33.27%), fatty acids (29.60%), and ethyl esters (9.23%), which are mainly responsible for the nutritional properties and odor of the fruit. This study presents an improved method for extracting volatile compounds, offering enhanced insights into the phytochemical composition, aroma, flavor, and bioactivity of jabuticaba.

Keywords: comprehensive two‐dimensional gas chromatography; direct immersion solid phase microextraction; hydrophilic microporous cartridge; jabuticaba; volatilome.