Platelet-rich fibrin associated to bovine bone induces bone regeneration in model of critical-sized calvaria defect of rats submitted to Zoledronic Acid therapy: PRF induces bone healing

Bárbara Gressy Duarte Souza Carneiro; Thays Allane Cordeiro Maia; Vanessa Costa; Simone Peixe Frieddrichsdorf; Delane Viana Gondim; Karuza Maria Alves Pereira; Mirna Marques Bezerra; Paula Goes

doi:10.1016/j.jormas.2024.102175

Platelet-rich fibrin associated to bovine bone induces bone regeneration in model of critical-sized calvaria defect of rats submitted to Zoledronic Acid therapy: PRF induces bone healing

J Stomatol Oral Maxillofac Surg. 2024 Dec 2:102175. doi: 10.1016/j.jormas.2024.102175. Online ahead of print.

Authors

Bárbara Gressy Duarte Souza Carneiro¹, Thays Allane Cordeiro Maia², Vanessa Costa³, Simone Peixe Frieddrichsdorf⁴, Delane Viana Gondim⁵, Karuza Maria Alves Pereira⁵, Mirna Marques Bezerra⁶, Paula Goes⁷

Affiliations

¹ Postgraduate program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil.
² Postgraduate program in Translational Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
³ Postgraduate program in Morphology, Faculty of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
⁴ Postgraduate program in Biomaterials and Oral Biology, University of São Paulo, São Paulo, SP, Brazil.
⁵ Department of Morphology, Faculty of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
⁶ Faculty of Medicine, Federal University of Ceará, Sobral, CE, Brazil.
⁷ Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil. Electronic address: paulagpinheiro@yahoo.com.br.

PMID: 39631530
DOI: 10.1016/j.jormas.2024.102175

Abstract

Purpose: Reconstruction of bone defects prior to implant installation is a challenge, especially when the patient uses bisphosphonates. Given this difficulty, many studies investigate biomaterials that can improve the bone regeneration process. In this context, this study aimed to investigate the effect of platelet-rich fibrin (PRF) and Bio-Oss (BO) on bone regeneration of rats submitted to critical-sized calvaria defects and treated with ZA.

Methods: Thirty Wistar rats received a single dose of ZA (120 μg/kg) and after 7 days, were submitted to an 8 mm calvaria defect. The animals were divided into 5 groups (n = 6): ZA, BO, PRF or BO+PRF; animals from control group did not receive ZA. All animals were euthanized 12 weeks after surgical procedure and calvaria collected to histological, histomorphometric and micro-CT analyses.

Results: BO+PRF increased the number of osteoblasts (33 %) and osteoclasts (58 %), as well as blood vessels (70 %) and Type I collagen (52 %) (p < 0.05) compared to ZA group.

Conclusion: In summary, the association of BO+PRF improved bone healing of large bone defect in rats receiving ZA and this may be an interesting approach for the treatment to be tested in patients under anti-resorptive therapy.

Keywords: Antiresorptive drugs; Bone density conservation agents; Bone regeneration; Platelet-rich fibrin.