Copper ions and iron ions are abundant metal ions in nature, and are also essential trace elements in the human body, playing a crucial role in various physiological activities. However, the detection of copper and iron ions also poses significant challenges. This article reports the synthesis of a novel fluorescent probe X2 based on the pyrazole structure, which exhibits unique optoelectronic properties, strong biological activity, good selectivity, excellent detection limit, high sensitivity, and its structure was characterized by 1H NMR, 13C NMR, and HR-ESI-MS. The obtained probe exhibits AIE characteristics, and its mechanism was analyzed through infrared spectrum and theoretical calculations. Finally, through cytotoxicity and cell imaging experiments, it was demonstrated that the probe has low toxicity and can be used in the field of biology. This article provides a new approach for detecting copper and iron ions using fluorescent probes with pyrazole structures.
Keywords: Cytotoxicity; DFT calculation; Fluorescent probe; Metal ion recognition; Pyrazole.
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