Three water isolates were previously identified as promising antibiotic producers from freshwater sources in Wisconsin, United States. Each isolate produced effective antibiotics against three or more bacterial relatives of antibiotic resistant pathogens. The isolates were identified as Calidifontibacillus erzuremensis , Pantoea agglomerans , and Pseudomonas glycinae through 16S rRNA sequencing and further characterized with biochemical tests to verify the genus and species of each isolate.
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