Antibody screening-assisted multichannel nanoplasmonic sensing chip based on SERS for viral screening and variants identification

Yi Liu; Huanjiao Weng; Zhiwei Chen; Ming Zong; Shubin Fang; Zili Wang; Shaohua He; Yangmin Wu; Jizhen Lin; Shangyuan Feng; Duo Lin

doi:10.1016/j.bios.2024.117015

Antibody screening-assisted multichannel nanoplasmonic sensing chip based on SERS for viral screening and variants identification

Biosens Bioelectron. 2024 Dec 2:271:117015. doi: 10.1016/j.bios.2024.117015. Online ahead of print.

Authors

Yi Liu¹, Huanjiao Weng², Zhiwei Chen³, Ming Zong⁴, Shubin Fang², Zili Wang², Shaohua He¹, Yangmin Wu¹, Jizhen Lin⁵, Shangyuan Feng⁶, Duo Lin⁷

Affiliations

¹ Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, 350117, China.
² The Cancer Center, Union Hospital, Fujian Medical University, Fuzhou, Fujian, 350001, China.
³ Fuzhou Center for Disease Control and Prevention, Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou, 350000, China.
⁴ Department of Clinical Laboratory, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.
⁵ The Cancer Center, Union Hospital, Fujian Medical University, Fuzhou, Fujian, 350001, China. Electronic address: linjizhen@fjmu.edu.cn.
⁶ Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, 350117, China. Electronic address: syfeng@fjnu.edu.cn.
⁷ Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, 350117, China. Electronic address: duo@fjnu.edu.cn.

PMID: 39662175
DOI: 10.1016/j.bios.2024.117015

Abstract

The Omicron variants of SARS-CoV-2 have been spreading globally and have never disappeared from our sight, indicating that their coexistence with humans has become a fact, and monitoring its evolution and spread remains a current task. Although polymerase chain reaction (PCR) is the most commonly used virus detection method, it requires labor-intensive and time-consuming procedures in a laboratory setting. Herein, a multichannel nanoplasmonic sensing chip based on surface enhanced Raman spectroscopy (SERS) was developed for detecting N and S proteins, as well as IgG and IgM, related to SARS-CoV-2 Omicron variants. Through a self-screening process, specific antibodies for on-site and rapid identification of important variants of concern (VoCs) were obtained, and their binding was confirmed by protein structure analysis. The use of these S protein specific antibodies can accurately identify Omicron VoCs (BA. 5, BF.7，XBB.1.5) with the detection limit (LoD) of 0.16 pg/mL. Then, the proposed SERS array chip was integrated with a hand-held Raman spectrometer to successfully detect the Omicron subvariants in real saliva samples within only 20 min, greatly reducing the detection time of PCR. This sensing technology will provide a powerful and rapid point-of-care testing (POCT) method for virus diagnosis, subtype identification, and post-infection antibody level monitoring.

Keywords: POCT; SERS; Sensing chip; Variants identification; Viral diagnosis.