Phase-transformed lactoferrin/strontium-doped nanocoatings enhance antibacterial, anti-inflammatory and vascularised osteogenesis of titanium

Jiali Wang; Jiaxin Guan; Fengzhen Jia; Zitong Tian; Lili Song; Lei Xie; Pengde Han; He Lin; Haixia Qiao; Xuejiao Zhang; Yong Huang

doi:10.1016/j.ijbiomac.2024.138608

Phase-transformed lactoferrin/strontium-doped nanocoatings enhance antibacterial, anti-inflammatory and vascularised osteogenesis of titanium

Int J Biol Macromol. 2024 Dec 9:287:138608. doi: 10.1016/j.ijbiomac.2024.138608. Online ahead of print.

Authors

Jiali Wang¹, Jiaxin Guan¹, Fengzhen Jia¹, Zitong Tian¹, Lili Song¹, Lei Xie², Pengde Han³, He Lin⁴, Haixia Qiao⁵, Xuejiao Zhang⁶, Yong Huang⁷

Affiliations

¹ College of Lab Medicine, Life Science Research Centre, Hebei North University, Zhangjiakou 075000, China.
² School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, China.
³ School of Materials Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China.
⁴ School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
⁵ College of Lab Medicine, Life Science Research Centre, Hebei North University, Zhangjiakou 075000, China. Electronic address: haixiaqiao@yeah.net.
⁶ College of Lab Medicine, Life Science Research Centre, Hebei North University, Zhangjiakou 075000, China. Electronic address: 527238610@qq.com.
⁷ College of Lab Medicine, Life Science Research Centre, Hebei North University, Zhangjiakou 075000, China. Electronic address: hycd08@126.com.

PMID: 39662560
DOI: 10.1016/j.ijbiomac.2024.138608

Abstract

Failure of orthopedic implants due to localized bacterial infections, inflammation and insufficient blood supply is always problematic. In this study, strontium-doped titanium dioxide nanotubes (STN) were firstly prepared on titanium surface, and then lactoferrin (LF) was loaded into strontium-doped nanotubes (STN) by the phase transition method, eventually the LF/TCEP-STN composite coating was successfully prepared. With the innate antimicrobial properties of LF, LF/TCEP-STN was effected against E. coli and S. aureus. Cellular assays showed that RAW264.7 (immune), HUVEC (angiogenic) and MC3T3-E1 (osteogenic) exhibited good adhesion and proliferative activity on the surface of LF/TCEP-STN. At the molecular level, LF/TCEP-STN modulated RAW264.7 polarization toward M2-type while promoting MC3T3-E1 differentiation toward osteogenesis. Meanwhile LF/TCEP-STN coating effectively promoted angiogenesis. The results of the bone defect model with or without infection demonstrated that the LF/TCEP-STN material had good anti-inflammatory, antibacterial, and vascularization-promoting osteogenesis. In addition, LF/TCEP-STN offered excellent blood compatibility and biosafety. As a multifunctional coating on implant surfaces, the study's results highlighted the viability of LF/TCEP-STN and offered fresh concepts for the clinical design of next-generation artificial bone implants with antibacterial, anti-inflammatory, and osteogenic properties.

Keywords: Anti-inflammatory; Antibacterial; Lactoferrin; Osteogenesis; SrTiO(3); Vascularization.