Comparing the release of growth factors when partially demineralized tooth matrix is mixed with platelet rich fibrin and injectable platelet rich fibrin: An in-vitro observational study

B H Harshitha Gowda; Ravishankar Krishna; Srikari Srinivasan; Madhusudan Astekar

doi:10.4103/jomfp.jomfp_545_23

Comparing the release of growth factors when partially demineralized tooth matrix is mixed with platelet rich fibrin and injectable platelet rich fibrin: An in-vitro observational study

J Oral Maxillofac Pathol. 2024 Jul-Sep;28(3):415-421. doi: 10.4103/jomfp.jomfp_545_23. Epub 2024 Oct 15.

Authors

B H Harshitha Gowda¹, Ravishankar Krishna¹, Srikari Srinivasan², Madhusudan Astekar³

Affiliations

¹ Department of Prosthodontics and Crown and Bridge, Faculty of Dental Sciences, Ramaiah University of Applied Sciences Bangalore, Karnataka, India.
² Department of Automotive and Aerospace, Faculty of Engineering, Ramaiah University of Applied Sciences Bangalore, Karnataka, India.
³ Department of Oral Pathology and Microbiology, Bareilly International University, Institute of Dental Sciences, Bareilly, UP, India.

Abstract

Context: Platelet concentrates are rich in growth factors that assist in regenerative medicine to promote healing and tissue regeneration. Similarly, partially demineralized tooth is a storehouse of many growth factors, assisting in bone regeneration. Hence, the present study aimed to quantify the release of growth factors from different platelet concentrates individually and when mixed with a partially demineralized tooth matrix.

Method: Human growth factors such as bFGF, EGF, PDGF-AB, TGF-beta-1, and VEGF-A present in platelet-rich fibrin and injectable platelet-rich fibrin from young and old male and female donors were quantified separately. Then these platelet concentrates were then mixed with a partially demineralized tooth matrix, which was powdered using a Smart Dentin Grinder. An enzyme-linked immunosorbent assay was used for the quantification of growth factors.

Results: The release of growth factors, such as platelet-rich fibrin and injectable platelet-rich fibrin, was not statistically significant; however, it was significantly greater when i-PRF was mixed with a partially demineralized tooth matrix.

Conclusion: The study revealed that the expression of growth factors was significantly greater when a partially demineralized tooth matrix was mixed with injectable platelet-rich fibrin than when combined with platelet-rich fibrin.

Keywords: Growth factors; I-PRF; partially demineralized tooth matrix; platelet concentrates; platelet rich fibrin.