Circular RNA NINL accelerates the malignant progression of cervical cancer

ChengCheng Cao; HaiFeng Zhang; Ting Zhang; CuiCui Nie

doi:10.1007/s12672-024-01588-8

Circular RNA NINL accelerates the malignant progression of cervical cancer

Discov Oncol. 2024 Dec 18;15(1):766. doi: 10.1007/s12672-024-01588-8.

Authors

ChengCheng Cao¹, HaiFeng Zhang¹, Ting Zhang², CuiCui Nie³

Affiliations

¹ Department of Gynecology, Affiliated Hospital of Shandong Second Medical University, Shandong, China.
² Department of Gynecology and Obstetrics, Sunshine Union Hospital, No. 9000, Yingqian Street, High-Tech Zone, Weifang, 261000, Shandong, China.
³ Department of Gynecology and Obstetrics, Sunshine Union Hospital, No. 9000, Yingqian Street, High-Tech Zone, Weifang, 261000, Shandong, China. niecuicui-suh@sinosig.com.

Abstract

Objective: The study's aim was to explore a novel miRNA/mRNA network mediated by circNINL in cervical cancer (CC).

Methods: Tumor tissue specimens and normal tissue specimens of 86 CC patients were collected. CircNINL, miR-2467-3p, and specificity protein 1 (SP1) expression levels in tissues were detected. In Hela cells, transfection was implemented to determine whether CircNINL, miR-2467-3p and SP1 regulated cellular progression. The interaction between circNINL, miR-2467-3p, and SP1 was explored.

Results: CircNINL and SP1 were abnormally increased whereas miR-2467-3p expression was suppressed in CC. Functionally, circNINL silencing or miR-2467-3p overexpression reduced CC cell progression, whereas circNINL overexpression did the opposite. CircNINL had a spongy effect on miR-2467-3p to regulate SP1 levels. miR-2467-3p inhibition or SP1 overexpression offset the inhibitory effect of circNINL silence on CC malignant progression.

Conclusion: CircNINL silencing can increase miR-2467-3p, thereby inducing the downregulation of SP1, thereby suppressing CC progression.

Keywords: Cervical cancer; CircNINL; Specificity protein 1; miR-2467-3p.