Integrated approach for cellulosic ethanol and succinic acid production: Gamma valerolactone-based pretreatment and co-fermentation of peanut shells

Chao Xu; Wen Zhou; Zuohua Zhu; Siran Feng; Fang Fang; Dandan Liu; Xudong Liu; Shushi Huang; Qian Lin; Yuande Peng; Chunliang Xie

doi:10.1016/j.ijbiomac.2024.138757

Integrated approach for cellulosic ethanol and succinic acid production: Gamma valerolactone-based pretreatment and co-fermentation of peanut shells

Int J Biol Macromol. 2024 Dec 16:138757. doi: 10.1016/j.ijbiomac.2024.138757. Online ahead of print.

Authors

Chao Xu¹, Wen Zhou¹, Zuohua Zhu², Siran Feng³, Fang Fang¹, Dandan Liu⁴, Xudong Liu⁵, Shushi Huang⁶, Qian Lin⁷, Yuande Peng², Chunliang Xie⁸

Affiliations

¹ School of Food and Bioengineering, Changsha University of Science and Technology, Changsha 410005, China.
² Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Key Laboratory of Biological and Processing for Bast Fiber Crops of Ministry of Agriculture and Rural Affairs, Engineering and Technology Center for Bast Fiber Crops of Hunan Province, Changsha 410205, China.
³ School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.
⁴ School of Landscape and Ecologocal Engineering, Hebei University of Engineering, Handan 056000, China.
⁵ State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, China.
⁶ Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning 530007, China.
⁷ Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Key Laboratory of Biological and Processing for Bast Fiber Crops of Ministry of Agriculture and Rural Affairs, Engineering and Technology Center for Bast Fiber Crops of Hunan Province, Changsha 410205, China; Hunan Provincial Key Laboratory of the Traditional Chinese Medicine Agricultural Biogenomics, China.
⁸ Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Key Laboratory of Biological and Processing for Bast Fiber Crops of Ministry of Agriculture and Rural Affairs, Engineering and Technology Center for Bast Fiber Crops of Hunan Province, Changsha 410205, China. Electronic address: xiechunliang@caas.cn.

PMID: 39694351
DOI: 10.1016/j.ijbiomac.2024.138757

Abstract

The inability to utilize pentose poses as a significant limitation to the production of cellulosic ethanol. To attain efficient raw material conversion and mitigate carbon dioxide emissions during cellulosic ethanol synthesis, a integrated approach focused on the co-processing of ethanol and succinic acid (SA) from peanut shells was proposed. The results demonstrated that the GVL system, containing 30 % water and catalyzed by dilute sulfuric acid, exhibited remarkable efficiency in pretreatment, boosting glucose yield sixfold relative to the untreated raw material. Under optimal conditions of 82 mM sulfuric acid, 141 °C, 56 min, and a solid-to-liquid ratio of 0.07, the glucose yield of the pretreated peanut shell reached 79.0 ± 0.22 %. Through recycling the pretreatment solvent, Tween 80-assisted enzymatic catalysis, and coupling of saccharification and co-fermentation processes, the complete utilization of lignocellulosic feedstocks and sustainable production of high titers of SA (86.1 g/kg) and ethanol (66.4 g/kg) were achieved. This study developed a novel integrated procedure for the efficient co-production of SA and ethanol from peanut shells, offering a new perspective for the efficient biorefinery of lignocellulosic biomass.

Keywords: Cellulosic ethanol; Lignocellulosic biomass; Succinic acid.