Red emitting fluorescence sensor based on Eu-tungstate complex for the detection of bilirubin in biological fluid

Ali Ibrahim Shkhair; Anju S Madanan; Susan Varghese; Merin K Abraham; Geneva Indongo; Greeshma Rajeevan; B K Arathy; Sara Muneer Abbas; Sony George

doi:10.1016/j.saa.2024.125572

Red emitting fluorescence sensor based on Eu-tungstate complex for the detection of bilirubin in biological fluid

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 9:329:125572. doi: 10.1016/j.saa.2024.125572. Online ahead of print.

Authors

Ali Ibrahim Shkhair¹, Anju S Madanan², Susan Varghese², Merin K Abraham², Geneva Indongo², Greeshma Rajeevan², B K Arathy², Sara Muneer Abbas¹, Sony George³

Affiliations

¹ Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, India; College of Food Science, Al-Qasim Green University, Babylon 51013, Iraq.
² Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, India.
³ Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, India; International Inter-University Centre for Sensing and Imaging (IIUCSI), Department of Chemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, India. Electronic address: emailtosony@gmail.com.

PMID: 39700550
DOI: 10.1016/j.saa.2024.125572

Abstract

Bilirubin is an important biomarker indicative of human health metabolism, especially in jaundice disease. In this work, we develop an Eu (Europium)-tungstate complex-based turn-off luminescence detection method for bilirubin in human (urineand serum)samples. The Eu-tungstate complex was synthesized by a straightforward one-pot procedure at ambient temperature, utilizing Europium chloride and Sodium tungstate as the precursors. The Eu-tungstate complex exhibited a very strong red emission and demonstrated excellent stability. The fluorescence of the Eu-tungstate complex in aqueous mediacan be turned off regularly by the gradual addition of bilirubin with a limit of detection (LoD) of 0.27 μM and alimit of quantitative (LoQ) of 0.91 μM.Moreover, this sensing technology can be utilized to sense bilirubin in actual samples.

Keywords: Bilirubin detection; Biosensor; Fluorescence; Lanthanide complex; Luminescence; Red emission.