Copper-based biomimetic nanozymes with multi-enzyme activity for phosphate detection

Zhendong Fu; TongJia Zhang; Cong Chen; Xiaoyu Wang; Liping Wang

doi:10.1016/j.saa.2024.125599

Copper-based biomimetic nanozymes with multi-enzyme activity for phosphate detection

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 15:329:125599. doi: 10.1016/j.saa.2024.125599. Online ahead of print.

Authors

Zhendong Fu¹, TongJia Zhang¹, Cong Chen¹, Xiaoyu Wang¹, Liping Wang²

Affiliations

¹ National Engineering Laboratory for AIDS Vaccine, Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
² National Engineering Laboratory for AIDS Vaccine, Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. Electronic address: wanglp@jlu.edu.cn.

PMID: 39700553
DOI: 10.1016/j.saa.2024.125599

Abstract

Nanozymes are nanoparticles with enzymatic activity, which are widely used in environmental and antibacterial research. Herein, we designed and synthesized novel amorphous nanozyme Cu-Im NPs with multiple enzyme-mimicking activities. Cu-Im NPs have the same active sites as natural laccase. In addition, the active center is similar to that of carbonic hydrolase, replacing the zinc ions with copper ions. Meanwhile, Cu-Im NPs also possess peroxidase-like activity. We reveal the multi-enzyme catalytic mechanisms of Cu-Im NPs. Notably, phosphate inhibits the laccase-like and peroxidase-like activities of Cu-Im NPs while activating their hydrolase activity. Based on these findings, we have developed a sensitive and selective method for detecting phosphate anions.

Keywords: Bio-mimic design; Carbonic anhydrase; Laccase; Nanozyme; Phosphate detection.