Integrative transcriptomic, proteomic and metabolomic analyses yields insights into muscle fiber type in cattle

Xiaofan Tan; Ruixue Zhao; Jing Chen; Zhiwei Yan; Xin Sui; Heling Li; Qiao Li; Xuehai Du; Yangzhi Liu; Siming Yao; Ying Yang; David M Irwin; Bojiang Li; Shuyi Zhang

doi:10.1016/j.foodchem.2024.142479

Integrative transcriptomic, proteomic and metabolomic analyses yields insights into muscle fiber type in cattle

Food Chem. 2024 Dec 13:468:142479. doi: 10.1016/j.foodchem.2024.142479. Online ahead of print.

Authors

Xiaofan Tan¹, Ruixue Zhao¹, Jing Chen¹, Zhiwei Yan¹, Xin Sui¹, Heling Li¹, Qiao Li¹, Xuehai Du², Yangzhi Liu³, Siming Yao², Ying Yang¹, David M Irwin⁴, Bojiang Li⁵, Shuyi Zhang⁶

Affiliations

¹ College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
² Liaoning Agricultural Development Service Center, Shenyang 110032, China.
³ Wellhope Foods Company Limited, Shenyang 110164, China.
⁴ Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
⁵ College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: bojiangli@syau.edu.cn.
⁶ College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: szhang@syau.edu.cn.

PMID: 39706111
DOI: 10.1016/j.foodchem.2024.142479

Abstract

Muscle fiber is an important factor in beef quality. Here, we compared fast-type longissimus dorsi muscle and slow-type psoas major muscle from cattle using transcriptomic, proteomic and metabolomic analyses. A total of 1717 differentially expressed genes (DEGs), 297 differentially abundant proteins (DAPs) and 193 differentially abundant metabolites (DAMs) were identified between LD and PM tissue, respectively. For verification, we selected 10 DEGs for qRT-PCR and 6 DAPs for western blotting, and showed they were consistent between the two approaches. GO and KEGG enrichment analyses revealed that some DEGs, DAPs and DAMs were enriched in muscle fiber type-associated GO terms and pathways. Many of them are involved in glycolysis, TCA and fatty acid metabolism. Integrated multi-omics analysis showed a correlation coefficient of 0.6244 between the transcriptome and proteome. This study provides a new understanding of molecular mechanisms involved in the determination of bovine muscle fiber type and meat quality.

Keywords: Cattle; DAMs; DAPs; DEGs; Muscle fiber.