Description of Massilia orientalis sp. nov., Isolated from Rhizosphere Soil of Ageratina adenophora

Abstract

A novel bacteria strain, designated YIM B02787^T, was isolated from rhizosphere soil of Ageratina adenophora, in Yunnan, southwest China. The strain was aerobic, Gram-stain-negative, rod-shaped and motile with one polar flagellum. Growth occurred at 4-45 °C (optimum, 20-30 °C) and pH 6.0-10.0 (optimum, 7.0-8.0), and in presence of 0-1% (w/v) NaCl. Phylogenetic analyses based on both 16S rRNA gene and genome sequences data revealed that strain YIM B02787^T belongs to the genus Massilia, being closely related to Massilia phosphatilytica KCTC 52513^T (98.93% similarity), M. putida KCTC 42761^T (98.86%), and M. kyonggiensis JCM 19189^T (98.78%). The DNA G+C content was 65.9%. The digital DNA-DNA hybridization and average nucleotide identity values between the isolate strain and aforementioned closely neighbors were low, at 35.8-48.9 and 88.5-92.5%, respectively. Strain YIM B02787^T contained Q-8 as the ubiquinone and major fatty acids were summed feature 3 (C_16:1ω7c and/or C_16:1ω6c, 45.5%) and C_16:0 (27.5%). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, two unidentified phospholipids, two unidentified aminophospholipids, and one unidentified polar lipid. On the basis of its phylogenetic, phenotypic and chemotaxonomic characteristics, strain YIM B02787^Trepresents a novel species of the genus Massilia, for which the name Massilia orientalis sp. nov. is proposed. The type strain is YIM B02787^T (= NBRC 116628^T = CGMCC 1.61539^T).