Host preference of Anopheles stephensi mosquitoes for blood feeding in south of Iran: Insights from Multiplex-PCR analysis

Mohammad Ali Oshaghi; Madineh Abbasi; Ahmad Ali Hanafi-Bojd

doi:10.1111/tmi.14076

Host preference of Anopheles stephensi mosquitoes for blood feeding in south of Iran: Insights from Multiplex-PCR analysis

Trop Med Int Health. 2024 Dec 20. doi: 10.1111/tmi.14076. Online ahead of print.

Authors

Mohammad Ali Oshaghi¹, Madineh Abbasi^{1

2}, Ahmad Ali Hanafi-Bojd^{1

3}

Affiliations

¹ Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
² Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
³ Zoonoses Research Center, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 39707593
DOI: 10.1111/tmi.14076

Abstract

Background: The study aims to determine the host preference for blood feeding among potential hosts of Anopheles stephensi in Iran, using the Multiplex-PCR method. An. stephensi is the primary malaria vector in urban areas of South Asia and the Middle East, including southern Iran, where approximately 30.21% of malaria cases are urban. This trend has become more evident during the recent outbreaks in Iran, driven by infections of Plasmodium falciparum, Plasmodium vivax, and as well as mixed infections. Hormozgan province, one of the most endemic areas in Iran, was selected for its critical public health significance. This study builds on the validated efficiency of Multiplex-PCR for blood meal analysis by applying it to mosquitoes in southern Iran.

Methods: In 2021, mosquitoes were collected monthly from three coastal villages in Bandar Abbas county, Hormozgan province, using WHO-recommended collection methods. Blood-fed An. stephensi mosquitoes were dissected, and their stomach contents analysed via Multiplex-PCR to identify human and animal blood sources.

Results: Of 77 An. stephensi samples analysed, humans were the most common host was humans (29.9%), followed by mammals (19%), dogs (2.6%), and birds (1.3%). Mixed blood meals were detected in 34% of samples, including 23% with human and other hosts. Informal observations suggest that domestic animals such as goats, sheep, and chickens are commonly present near homes in these areas.

Conclusion: Approximately 50% of An. stephensi blood meals were sourced from humans, with 29% exclusively from humans and 23% from mixed hosts. Domestic animals such as goats, sheep, and chickens appear to attract mosquitoes, highlighting their potential role in malaria dynamics. Zooprophylaxis, alongside existing measures like insecticide residual spraying, insecticide-treated bed nets, and personal protection strategies, may strengthen urban malaria control. Further research on the ecological and behavioural drivers of mosquito host selection in urban settings is warranted.

Keywords: Anopheles stephensi; Iran; Multiplex‐PCR; blood‐feeding hosts.