A saccharides regulated fluorescence ratio sensing array for bacterial recognition based on lectin response

Abstract

Array sensing employs cross-identification among analytes and various sensing units to identify substances or complex systems. This manuscript presents a fluorescence ratio sensing array based on lectin responses for the accurate identification of different bacteria. This strategy uses a saccharide-sensitive polymer as the sensing unit within the sensor. By incorporating various saccharides, it regulates the properties of the single sensing unit at the molecular level, altering its interaction with the analyte. This modulation leads to the generation of multiple distinct detection signals for the target, effectively facilitating the goal of array sensing. This approach streamlines the design and construction of the array sensor, while simultaneously enhancing detection efficiency. Not only does this sensing strategy achieve the differentiation and quantification of various types of lectins, but it also enables the identification of different bacterial species based on their unique lectin response profiles. This research introduces a novel approach that simplifies the construction of array sensors and simultaneously furnishes a potent tool for diagnosing and assessing bacterial infections within clinical settings.

Keywords: Array sensing; Bacteria; Fluorescence ratio; Lectin; Pattern recognition.