The effect of Licochalcone A on proliferation, invasion, and drug resistance of glioma cells by regulating TLR4/NF-κB signaling pathway

Baigui Zhou; Kun Mu; Xuzhou Yu; Xu Chen; Xiaoying Shi

doi:10.1016/j.clinsp.2024.100542

The effect of Licochalcone A on proliferation, invasion, and drug resistance of glioma cells by regulating TLR4/NF-κB signaling pathway

Clinics (Sao Paulo). 2024 Dec 20:80:100542. doi: 10.1016/j.clinsp.2024.100542. Online ahead of print.

Authors

Baigui Zhou¹, Kun Mu², Xuzhou Yu³, Xu Chen², Xiaoying Shi⁴

Affiliations

¹ Department of Neurology, Zhejiang Jinhua Guangfu Tumor Hospital, Zhejiang, PR China. Electronic address: jhw0756@163.com.
² Department of Neurology, Zhejiang Jinhua Guangfu Tumor Hospital, Zhejiang, PR China.
³ Department of Respiratory Oncology, Zhejiang Jinhua Guangfu Tumor Hospital, Zhejiang, PR China.
⁴ Department of Emergency, Zhejiang Jinhua Guangfu Tumor Hospital, Zhejiang, PR China.

PMID: 39708583
DOI: 10.1016/j.clinsp.2024.100542

Abstract

Objective: Based on Toll Like Receptor 4 (TLR4)/Nuclear Factor-κB (NF-κB) Exploring the effects of Licochalcone A (LCA) on the proliferation, invasion, and drug resistance of glioma cells through signaling pathways.

Methods: Cultivate human glioma cell line U251 in vitro, induce drug-resistant cell line U251/TMZ with Temozolomide (TMZ), and validate the results. Different concentrations of licorice chalcone A were used to treat U251 cells and U251/TMZ cells, and were named as control group, low-dose group, medium-dose group, and high-dose group, respectively. CCK-8 assay, cell adhesion assay, and Transwell assay were used to detect cell survival rate, cell adhesion rate, number of migrating cells, and number of invading cells, respectively.

Results: The cell survival rate, cell adhesion rate, number of migrating and invading cells in the high-dose group were lower than those in the medium-dose group and lower than those in the control group. High-dose group TLR4, NF-κB mRNA and protein levels were lower than those in the medium dose group and lower than those in the control group (p < 0.05). Compared with the si-NC group, the si-TLR4 group showed a decrease in cell survival rate and adhesion rate, as well as a decrease in the number of migrating and invading cells, the levels of CyclinD1 and N-cadherin proteins decreased, while the levels of E-cadherin protein increased (p < 0.05).

Conclusion: LCA could inhibit the proliferation and metastasis of glioma cells and reverse drug resistance, possibly by inhibiting the TLR4/NF-κB signaling pathway.

Keywords: Cell proliferation; Drug resistance; Glioma of the brain; LCA; Nuclear factor κB; Toll like receptor-4.