Development of a visual immunosensing platform based on fluorescent microspheres for the rapid detection of ochratoxins in cereals

Xiaofang Li; Maozhong Sun; Liguang Xu; Liqiang Liu; Xinxin Xu; Hua Kuang; Chuanlai Xu

doi:10.1016/j.foodchem.2024.142510

Development of a visual immunosensing platform based on fluorescent microspheres for the rapid detection of ochratoxins in cereals

Food Chem. 2024 Dec 16:469:142510. doi: 10.1016/j.foodchem.2024.142510. Online ahead of print.

Authors

Xiaofang Li¹, Maozhong Sun¹, Liguang Xu¹, Liqiang Liu¹, Xinxin Xu², Hua Kuang¹, Chuanlai Xu¹

Affiliations

¹ International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, PR China.
² International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, PR China. Electronic address: xuxinxin@jiangnan.edu.cn.

PMID: 39708639
DOI: 10.1016/j.foodchem.2024.142510

Abstract

Ochratoxins (OTs, including OTA, OTB, and OTC), exhibit nephrotoxicity, carcinogenicity, teratogenicity, and immunotoxicity, contaminating wheat, maize, and rice, so endangering human health. In this study, we designed and screened haptens using computational chemistry to prepare mAb 4G11, which simultaneously recognized OTA, OTB, and OTC with high sensitivity and specificity. We developed a fluorescent microsphere immunochromatographic assay (FMIA), which could detect the total amount of OTs with a visual detection limit of 0.5 ng/mL and a quantification limit of 0.27 ng/mL. Recovery experiments ranged from 95.94 % to 106.01 % with coefficients of variation ranging from 2.74 % to 6.30 %. The results obtained from the FMIA were found to be in alignment with those obtained from liquid chromatography-tandem mass spectrometry. The FMIA reliability was further demonstrated using real samples. FMIA can serve as a valuable instrument for the surveillance of the content of OTs in cereals.

Keywords: Cereals; Computational chemistry; Fluorescent microsphere immunochromatography; Ochratoxins.