Thermosensitive, injectable, antibacterial glabridin liposome/chitosan dual network hydrogel for diabetic wound healing

Yuyang Cao; Duoling Xu; Zhaoji Zu; Genlu Xu; Yixi Zeng; Jiyu Weng; Zhenhai Huang; Hongping Xiang; Lanyue Zhang; Jinlong Ding; Hui Li

doi:10.1016/j.ijbiomac.2024.138966

Thermosensitive, injectable, antibacterial glabridin liposome/chitosan dual network hydrogel for diabetic wound healing

Int J Biol Macromol. 2024 Dec 19:290:138966. doi: 10.1016/j.ijbiomac.2024.138966. Online ahead of print.

Authors

Yuyang Cao¹, Duoling Xu², Zhaoji Zu³, Genlu Xu¹, Yixi Zeng¹, Jiyu Weng¹, Zhenhai Huang¹, Hongping Xiang³, Lanyue Zhang⁴, Jinlong Ding¹, Hui Li⁵

Affiliations

¹ School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China.
² Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China; Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, 21205, USA.
³ School of Materials and Energy, Guangdong University of Technology, Guangzhou 510006, China.
⁴ School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. Electronic address: zhanglanyue@gdut.edu.cn.
⁵ School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China; Department of Traditional Chinese Medicine, Institute of Guangdong Geriatric, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China.

PMID: 39708855
DOI: 10.1016/j.ijbiomac.2024.138966

Abstract

Thermosensitive hydrogels show great potential in healing diabetic wounds, but they are still challenged by the long healing time, risk of infectivity, and accumulation of melanin. Herein, a dual network hydrogel is designed, which consists of chlorogenic acid (CA) modified chitosan (CS) (CA@CS), poly(N-isopropylacrylamide) (PNIPAm), and glabridin liposomes (GL). The gelation transition temperature of the hydrogel is 32-34 °C, which thus endows it with superior injectability at ambient temperature. Moreover, GL/PNIPAm/CA@CS also exhibits excellent biocompatibility, and can promote the growth of epidermal cells, and the healing of diabetic wounds. GL/PNIPAm/CA@CS can also control the immune reactions by enhancing the release of CD206, and decreasing the formation of CD86 and ROS, which further promotes the production of CD31 and VEGF, and reduces the expression of pro-inflammatory factors, thus aiding in the healing of diabetic wounds. Furthermore, GL/PNIPAm/CA@CS can also suppress the growth bacterial, which can thus decrease the wound microbiota levels and facilitate the recovery of diabetic wounds. More importantly, it can reduce melanin production by 80 % due to the action of glabridin. Consequently, GL/PNIPAm/CA@CS shows significant promise in enhancing the wound healing in future and decreasing the accumulation of melanin.

Keywords: Diabetic wound healing; Dual network; Glabridin liposome; Thermosensitive hydrogel.