Generation of induced pluripotent stem cell line, NCHi027-A, from a female child with Posterior Cloaca (Type B)

Cintia E Gomez Limia; Karunya Albert; Chaitrali Atre; Kusum Basnet; Darria L Streeter; Nathalia G Amado; Linda A Baker

doi:10.1016/j.scr.2024.103636

Generation of induced pluripotent stem cell line, NCHi027-A, from a female child with Posterior Cloaca (Type B)

Stem Cell Res. 2024 Dec 19:82:103636. doi: 10.1016/j.scr.2024.103636. Online ahead of print.

Authors

Cintia E Gomez Limia¹, Karunya Albert¹, Chaitrali Atre¹, Kusum Basnet¹, Darria L Streeter¹, Nathalia G Amado², Linda A Baker³

Affiliations

¹ Kidney and Urinary Tract Center, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA.
² Kidney and Urinary Tract Center, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA. Electronic address: Nathalia.amado@nationwidechildrens.org.
³ Kidney and Urinary Tract Center, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA. Electronic address: linda.baker1@nationwidechildrens.org.

PMID: 39709757
DOI: 10.1016/j.scr.2024.103636

Abstract

Cloaca is an ultra-rare severe anorectal malformation in females where the gastrointestinal, genital, and urologic systems converge. Posterior Cloaca (Type B) is an extremely rare specific variant, where the urogenital sinus opens just anterior to the anus. NCHi027-A is an iPSC line derived from skin fibroblasts of a 4 year and 8-month-old female with Posterior Cloaca (Type B) using Sendai Virus reprogramming. This iPSC line shows typical iPSC morphology, has normal karyotype, expresses pluripotency markers, and can be differentiated into three germ layers.