A fluorescent signal amplification strategy via host-guest recognition for cortisol detection

Jiao Wang; Jinming Kong; Xueji Zhang

doi:10.1016/j.saa.2024.125611

A fluorescent signal amplification strategy via host-guest recognition for cortisol detection

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 19:329:125611. doi: 10.1016/j.saa.2024.125611. Online ahead of print.

Authors

Jiao Wang¹, Jinming Kong², Xueji Zhang³

Affiliations

¹ School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, PR China.
² School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, PR China. Electronic address: j.kong@njust.edu.cn.
³ School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, Guangdong 518060, PR China.

PMID: 39709860
DOI: 10.1016/j.saa.2024.125611

Abstract

Psychological stress is a major contributor to individual health disparities. Accurate and quantitative detection of stress markers is crucial preventing mental health related problems. Supramolecular chemistry is widely used in drug delivery, catalysis, sensors and other applications. However, due to the difficulty of host functionalization such as cyclodextrins and solid-state pillar[n], it is still a challenge to directly realize the detection of guests through host-guest recognition behavior. Here, we reported an atom transfer radical polymerization (ATRP) fluorescent biosensor for direct and selective detection of guest molecule stress marker cortisol, translating molecular recognition behavior into quantifiable detection signals. Realizes quantitative chemical detection and builds a portable and affordable sensing platform for quantitative detection of target molecules without complex cross-linking steps. Overcomes the disadvantages of traditional methods that require the use of antibodies or are difficult to functionalize during the host-guest recognition process. This ATRP fluorescent biosensor was fabricated by employing zinc phthalocyanine (ZnPc) as a photocatalyst under 630 nm wavelength radiation, β-CD-Br₁₅ as a macromolecular initiator, and fluorescein O-methacrylate (FMA-O) as a monomer for polymerization. The system provides ultra-high sensitivity for the detection of cortisol (limit of detection 0.47 ng/mL) and specificity for the detection of cortisol in the presence of interfering substances such as progesterone and urea. Selective and real sample experiments confirm the specificity and scalability of this mechanism can also be customized by the rational design of the host-guest complex to quantitatively detect various molecules. This study confirms the feasibility of using a cyclodextrin-centered macromolecular initiator as a capture and label-free fluorescent biosensor for cortisol, a stress biomarker.

Keywords: ATRP; Fluorescence sensing; Host-guest recognition; Stress biomarker.