Prevalence and molecular characterization of Campylobacter spp. isolated from chicken, beef, pork and sheep livers at Irish abattoirs

Deirdre M Prendergast; Richard O'Keeffe; Dayle Johnston; Joanne McLernon; Fiona Power; Brian Byrne; Montserrat Gutierrez

doi:10.1016/j.ijfoodmicro.2024.111029

Prevalence and molecular characterization of Campylobacter spp. isolated from chicken, beef, pork and sheep livers at Irish abattoirs

Int J Food Microbiol. 2024 Dec 18:430:111029. doi: 10.1016/j.ijfoodmicro.2024.111029. Online ahead of print.

Authors

Deirdre M Prendergast¹, Richard O'Keeffe², Dayle Johnston², Joanne McLernon², Fiona Power², Brian Byrne², Montserrat Gutierrez²

Affiliations

¹ Food Microbiology Division, Department of Agriculture, Food and the Marine, Backweston Laboratories, Celbridge, Co. Kildare, Ireland. Electronic address: deirdre.prendergast@agriculture.gov.ie.
² Food Microbiology Division, Department of Agriculture, Food and the Marine, Backweston Laboratories, Celbridge, Co. Kildare, Ireland.

PMID: 39709889
DOI: 10.1016/j.ijfoodmicro.2024.111029

Abstract

Thermotolerant Campylobacter spp. are the leading causes of food-borne diarrhoea in humans with most cases attributed to C. jejuni, and C. coli. Although chicken meat is considered the main source of infection in humans, the significance of other sources for campylobacteriosis in humans is less understood. The objective of this study was to determine the prevalence of thermotolerant Campylobacter spp. in chicken, beef, pork, and sheep liver in Ireland and to carry out whole genome sequencing (WGS) on the resulting isolates to characterize them molecularly. In addition, genome sequences of Irish clinical isolates were downloaded from the National Center for Biotechnology Information (NCBI) and compared with sequences from this study. The prevalence of Campylobacter spp. in chicken, beef, pork and sheep liver was 70 %, 4.4 %, 16 % and 80.0 %, respectively. Chi-Squared analysis indicated that the statistical differences in positivity rates between the four species were significant (P < 0.001). Amongst the 81 (43 %) positive liver samples, speciation revealed an overall predominance of C. jejuni (62 %), followed by C. coli (48 %) and C. lari (1 %) in all meat types except pork. Nine (11 %) samples were confirmed positive for more than one Campylobacter species with five of these nine samples recovered from sheep livers. Following analysis of WGS data, a wide range of diversity was observed and where clusters were identified, all were confined to the same animal species. No AMR genes were identified in the C. lari isolate, while C. jejuni and C. coli isolates were found to harbour resistance genes for tetracyclines, beta-lactams, aminoglycosides, and quinolones. Two clusters were identified between isolates from this study and human clinical data and the most prevalent clonal complex was CC-21, identified in this study and in clinical isolates. These results highlight the role of liver as a potential source of human Campylobacter infection. The significance of liver as a vehicle of human campylobacteriosis needs to be examined further particularly in respect to ovine sources.

Keywords: Antimicrobial resistance; Campylobacteriosis; Cluster; Food safety; Genomic diversity; Sequence types; Whole genome sequencing.