Revealing critical mechanisms involved in carbon nanosol-mediated tobacco growth using small RNA and mRNA sequencing in silico approach

Abstract

Nanomaterials have been shown to promote crop growth, yield and stress resistance. Carbon nanosol (CNS), a type of nanomaterial, is used to regulate tobacco shoot and root growth. However, information about the application of CNS to crop plants, especially tobacco, is still limited. Based on differential expression analysis and trend analysis, several miRNAs (miRN21-Novel-5p-mature, miR319b-Probable-5p-mature, miR160a-c-Known/Probable-5p-mature and miR156c-e-Known-5p-mature/star) and their target genes, including transcription factors (TFs), are likely responsible for the effect of CNS on promoting the growth of tobacco plants. In addition, we characterized nine TFs [Nitab4.5_00001789g0110 (NbbZIP), Nitab4.5_00001176g0010 (NbMYB), Nitab4.5_0001366g0010 (NbNAC), Nitab4.5_00000895g013 (NbMYB), Nitab4.5_0001225g0120 (NbNAC), Nitab4.5_0000202g0230 (NbDof), Nitab4.5_0002241g0010 (NbMYB-related), Nitab4.5_0000410g0060 (NbTCP), and Nitab4.5_0000159g0180 (NbC2H2)] associated with the response of tobacco to CNS according to the differential expression analysis, TF‒gene interaction network analysis and weighted correlation network analysis (WGCNA). Taken together, the findings of our study help understand CNS-mediated growth promotion in tobacco plants. The identification of candidate miRNAs and genes will provide potential support for the use of CNS in tobacco.

Keywords: Carbon nanosol; Growth; Tobacco; Transcription factor; miRNA.