Outcomes of DCD Liver Transplant Using Sequential Normothermic Regional Perfusion and Normothermic Machine Perfusion or NRP Alone Versus Static Cold Storage

Kristopher P Croome; Vijay Subramanian; Amit K Mathur; Bashar Aqel; Shennen A Mao; Jacob N Clendenon; Dana K Perry; Kiran Dhanireddy; C Burcin Taner

doi:10.1097/TP.0000000000005301

Outcomes of DCD Liver Transplant Using Sequential Normothermic Regional Perfusion and Normothermic Machine Perfusion or NRP Alone Versus Static Cold Storage

Transplantation. 2024 Dec 24. doi: 10.1097/TP.0000000000005301. Online ahead of print.

Authors

Kristopher P Croome¹, Vijay Subramanian², Amit K Mathur³, Bashar Aqel⁴, Shennen A Mao¹, Jacob N Clendenon¹, Dana K Perry¹, Kiran Dhanireddy², C Burcin Taner¹

Affiliations

¹ Department of Transplant, Mayo Clinic Florida, Jacksonville, FL.
² Transplant Institute, Tampa General Hospital, Tampa, FL.
³ Department of Surgery, Mayo Clinic Arizona, Phoenix, AZ.
⁴ Division of Gastroenterology and Hepatology, Mayo Clinic Arizona, Phoenix, AZ.

PMID: 39716357
DOI: 10.1097/TP.0000000000005301

Abstract

Background: The availability of in situ normothermic regional perfusion (NRP) or ex situ normothermic machine perfusion (NMP) has revolutionized donation after circulatory death (DCD) liver transplant (LT). While some have suggested that NRP and NMP may represent competing technologies for DCD LT, there are many scenarios where these technologies can function in a complementary manner.

Methods: Between January 2022 and March 2024, 83 DCD LTs were performed using NRP (62 NRP alone and 21 NRP + NMP) and were compared with 297 static cold storage (SCS) DCD LTs. NRP + NMP was used in scenarios with (1) long travel distances, (2) complicated transplant recipients, or (c) the need for additional liver graft recovery in "marginal" cases.

Results: Ischemic cholangiopathy was lower in the NRP alone group (0%) and the NRP + NMP group (0%) compared with the SCS group (16.8; P < 0.001 and P = 0.04, respectively). In addition, early allograft dysfunction, number of packed red blood cells transfused, and acute kidney injury were lower in the NRP alone and NRP + NMP groups compared with the SCS group. Graft survival was higher in cases where NRP was used than in cases where SCS was used (P = 0.016). In all the cases where lactate remained elevated at the end of NRP (mean 8.2 ± 2.0), it ultimately normalized at the end of NMP (0.92 ± 0.56).

Conclusions: The present study demonstrates lower rates of ischemic cholangiopathy and improved graft survival with NRP alone or NRP + NMP compared with SCS when using liver grafts from DCD donors. It also demonstrates that excellent outcomes can be achieved with sequential NRP + NMP in cases with prolonged travel distances, complicated recipients, or when there is a need for additional liver recovery in "marginal" cases.