GLR36T807I Mutation of Casuarina equisetifoli Is Associated With a Decreased JA Response to Insect Feeding by Lymantria xylina

Huan Chang; Chengli Li; Tengfei Zhu; Shouping Cai; Jie Chen; Fangfang Zhan; Liqiong Zeng; Yu Fang; Gongfu Ye; Jian Li; Jun Su

doi:10.1111/pce.15347

GLR36^T807I Mutation of Casuarina equisetifoli Is Associated With a Decreased JA Response to Insect Feeding by Lymantria xylina

Plant Cell Environ. 2024 Dec 24. doi: 10.1111/pce.15347. Online ahead of print.

Authors

Huan Chang¹, Chengli Li², Tengfei Zhu³, Shouping Cai⁴, Jie Chen⁴, Fangfang Zhan⁴, Liqiong Zeng⁴, Yu Fang⁵, Gongfu Ye⁴, Jian Li⁶, Jun Su¹

Affiliations

¹ Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China.
² Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China.
³ National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Taian, Shandong Province, China.
⁴ Fujian Academy of Forestry Sciences, Fuzhou, Fujian Province, China.
⁵ Institute of Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China.
⁶ Key Laboratory of Forest Ecosystem Process and Management of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China.

PMID: 39718115
DOI: 10.1111/pce.15347

Abstract

Lymantria xylina is the most important defoliator, damaging the effective coastal windbreak tree species Casuarina equisetifolia. However, the underlying genetic mechanisms through which C. equisetifolia responds to L. xylina attacks remain unknown. Here, we compared the transcriptional, phytohormone and metabolic differences between susceptible (S) and resistant (R) C. equisetifolia cultivars in response to L. xylina feeding. The main L. xylina-induced resistance in C. equisetifolia was a jasmonate (JA) response and JA synthesis was highly induced by L. xylina feeding at both the transcriptional and metabolic levels, thus promoting flavonoid accumulation. The JA response was highly activated by L. xylina feeding on the R but not in the S cultivar, although the JA signalling pathway was intact in both cultivars. We found a single amino acid mutation in the homologues of glutamate receptor-like protein 3.6 (CeGLR3.6^T807I) in the S cultivar. Compared with the GLR3.6 homologues in the R cultivar, phosphorylation of CeGLR3.6^T807I was not induced by insect feeding, leading to a decreased JA response in the S cultivar. Collectively, this study provides new insights into the function of CeGLR3.6 in regulating the JA response of C. equisetifolia to L. xylina feeding.

Keywords: Casuarina equisetifolia; Lymantria xylina; flavonoids; glutamate receptor‐like protein 3.6 (GLR3.6); jasmonic acid; phosphorylation.

Grants and funding

This work was supported by the National Natural Science Foundation of China (NSFC) (32071753), the Fujian Forestry Science and Technology Project (ZMGG-0704) and the Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University (72202200205).