Genome-wide characterization of RsHDAC gene members unravels a positive role of RsHDA9 in thermotolerance in radish (Raphanus sativus L.)

Weilan Zhang; Yingfei Ma; Yudi Huang; Min He; Xiaoli Zhang; Liang Xu; Yan Wang; Liwang Liu; Yuelin Zhu

doi:10.1016/j.plaphy.2024.109439

Genome-wide characterization of RsHDAC gene members unravels a positive role of RsHDA9 in thermotolerance in radish (Raphanus sativus L.)

Plant Physiol Biochem. 2024 Dec 20:219:109439. doi: 10.1016/j.plaphy.2024.109439. Online ahead of print.

Authors

Weilan Zhang¹, Yingfei Ma¹, Yudi Huang¹, Min He¹, Xiaoli Zhang¹, Liang Xu², Yan Wang¹, Liwang Liu¹, Yuelin Zhu³

Affiliations

¹ Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China) of MOAR, College of Horticulture, Sanya Institute, Nanjing Agricultural University, Nanjing 210095, PR China.
² Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China) of MOAR, College of Horticulture, Sanya Institute, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: nauxuliang@njau.edu.cn.
³ Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China) of MOAR, College of Horticulture, Sanya Institute, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: ylzhu@njau.edu.cn.

PMID: 39721191
DOI: 10.1016/j.plaphy.2024.109439

Abstract

Radish is an economically important root vegetable crop worldwide. Histone deacetylases (HDACs), one of the most important epigenetic regulators, play prominent roles in plant growth and development as well as abiotic stress responses. Nevertheless, the systematical characterization and critical roles of HDAC gene members in thermogenesis remains elusive in radish. Herein, a total of 21 RsHDAC genes were identified from the radish genome. Among them, two RsSRTs, six RsHDTs and 13 RsHDAs were classified into the SIR2, HD2 and RPD3/HDA1subfamily, respectively. The RNA-seq analysis indicated that three RsHDAs (RsHDA6.1, RsHDA6.2 and RsHDA19) and five RsHDTs exhibited high expression in vascular cambium of radish taproot. Both the RsHDT3 and RsHDA9 showed dramatically up-regulated expression under heat, salt and three heavy metals treatments. Moreover, the transient LUC reporter assay revealed that the promoter activity of the nucleus-localized RsHDA9 was intensely induced by heat stress. Intriguingly, overexpression of RsHDA9 promoted thermotolerance via enhancing proline accumulation and scavenging of reactive oxygen species in radish cotyledons, whereas the supplement of trichostatin A (TSA) led to the opposite phenotype. Notably, RsWRKY26 bound to the RsHDA9 promoter and activated its transcription to achieve enhancing thermotolerance in radish. Collectively, these findings would facilitate deciphering molecular mechanism underlying RsHDA9-mediated regulatory network of thermogenesis in radish.

Keywords: Heat stress; Raphanus sativus; RsHDA9; RsHDACs; RsWRKY26; Thermotolerance.