Highly catalytic CoFe-prussian blue analogue/ZIF-67 yolk-shell nanocube-decorated MBene nanosheets for ultrasensitive electrochemical cancer-specific neoantigen biosensor

Xinmei Song; Yuan Ming; Juan Liu; Bingying Jiang; Ruo Yuan; Yun Xiang

doi:10.1016/j.jcis.2024.12.155

Highly catalytic CoFe-prussian blue analogue/ZIF-67 yolk-shell nanocube-decorated MBene nanosheets for ultrasensitive electrochemical cancer-specific neoantigen biosensor

J Colloid Interface Sci. 2024 Dec 21;683(Pt 2):58-67. doi: 10.1016/j.jcis.2024.12.155. Online ahead of print.

Authors

Xinmei Song¹, Yuan Ming², Juan Liu¹, Bingying Jiang³, Ruo Yuan¹, Yun Xiang⁴

Affiliations

¹ Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
² School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, PR China.
³ School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, PR China. Electronic address: jiangby@cqut.edu.cn.
⁴ Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yunatswu@swu.edu.cn.

PMID: 39721408
DOI: 10.1016/j.jcis.2024.12.155

Abstract

Neoantigens exclusively presented by human leukocyte antigens (HLAs) on cancer cell surfaces are newly discovered and highly cancer-specific biomarkers for cancer diagnosis. The current available method for detecting neoantigens is predominantly based on Mass spectrometry with inevitable limitations of high cost, complexity and isotope labels. In this work, we describe the development of an innovative catalytic electrochemical biosensor for ultrasensitive detection of neoantigen in cell lysates. Such biosensor design involves the synthesis of new and highly catalytic CoFe-prussian blue analogue/ZIF-67 yolk-shell nanocube-decorated MBene nanosheets (CoFe-PBA/ZIF-67/MBene) and the derivatization of electrochemically inert neoantigen to electroactive molecule. The as synthesized CoFe-PBA/ZIF-67/MBene nanocomposite exhibits large specific surface area, excellent conductivity and abundant active sites for electrochemical oxidation of the derivatized neoantigens for the yield of considerably amplified currents for sensitive detection of target neoantigen with low to 0.047 nM detection limit. The biosensor can also be applied for monitoring low levels of HLA-presented neoantigen complexes in cell lysates, offering new insights into methodological advancements in neoantigen analyses for cancer research.

Keywords: Cancer-specific neoantigen; CoFe-PBA/ZIF-67 yolk-shell nanocube; Electrochemical biosensor; MBene nanosheet.