Lactic Acid Bacteria Surface Proteins in the Mechanisms of Cell Adhesion and Immunoregulation

Ziheng Meng; Xianqing Huang; Mingwu Qiao; Lianjun Song; Yufei Liu; Dan Hai

doi:10.1002/fsn3.4517

Lactic Acid Bacteria Surface Proteins in the Mechanisms of Cell Adhesion and Immunoregulation

Food Sci Nutr. 2024 Oct 30;12(12):10148-10163. doi: 10.1002/fsn3.4517. eCollection 2024 Dec.

Authors

Ziheng Meng¹, Xianqing Huang^{1

2}, Mingwu Qiao^{1

2}, Lianjun Song^{1

2}, Yufei Liu¹, Dan Hai^{1

2}

Affiliations

¹ College of Food Science and Technology Henan Agricultural University Zhengzhou China.
² Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control Zhengzhou China.

Abstract

This study delves into the role of lactic acid bacteria (LAB) surface proteins in cell adhesion and immunoregulation. Using fluorescence microscopy, we observed distinct adhesion patterns on various cell types. LAB surface proteins demonstrated concentration-dependent inhibition of Salmonella adhesion, with LAB69 exhibiting potent antagonistic effects. Genetic expression analysis revealed nuanced responses in key genes (MD2, TLR4, IL-10, MUC3, MIF) across different cell types, highlighting the diverse immunomodulatory effects of LAB surface proteins. Modulation of pro-inflammatory (TNF-α) and anti-inflammatory (IL-10) cytokines further emphasized the complex interplay. In conclusion, this study underscores the pivotal role of LAB surface proteins in mediating cell adhesion and immunoregulation, providing a foundation for isolating specific immunomodulatory molecules within LAB surface proteins for potential applications in microbial ecological agents.

Keywords: cell adhesion; immunomodulatory molecules; immunoregulation; lactic acid bacteria; surface proteins.