Light responses during early day phases of CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and PSEUDO-RESPONSE REGULATOR (PRR) homologous genes in the moss Physcomitrium patens

Abstract

Circadian clocks facilitate organisms' adaptation to the day-night environmental cycle. Some of the component genes of the clocks ("clock genes") respond directly to changes in ambient light, supposedly allowing the clocks to synchronize to and/or oscillate robustly in the environmental cycle. In the dicotyledonous model plant Arabidopsis thaliana, the clock genes CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), LATE ELONGATED HYPOCOTYL (LHY) and PSEUDO-RESPONSE REGULATOR 9 (PRR9) show transient expression in response to the morning light. Here we studied light responses of CCA1a/CCA1b and PRR2, homologous genes to CCA1/LHY and PRR9, respectively, in the moss Physcomitrium patens. We found that light of different wavelengths induced PRR2 while they repressed CCA1a/CCA1b. A disruption strain lacking all phytochrome genes lost PRR2 induction, but still maintained CCA1a/CCA1b repression. The remaining light repression of CCA1a/CCA1b was impaired by the photosynthesis inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Probably therefore, a phytochrome signaling induces PRR2, whereas a photosynthesis-mediated signaling represses CCA1a/CCA1b. Conservation and divergence in the clock gene responses between P. patens and A. thaliana are discussed.

Keywords: Physcomitrium patens; circadian clock; clock genes; photosynthesis; phytochrome.