The regional variation of the femur bone is influenced by sex, stature geographical area and heredity, as the environment plays an important role in the developmental process. This cross-sectional descriptive purposive study was done on 150 (64 right and 86 left) fully ossified dry human femur of Bangladeshi people to find out the variation of neck length on anterior and posterior aspect. Sample was collected in the Department of Anatomy, Mymensingh Medical College, Bangladesh from January 2019 to December 2019. Fractured, damaged and remodeled bones were excluded from the study. Neck length was measured with the help of digital Vernier slide calipers. The mean±SD neck length on anterior aspect of femur on right and left side was, 30.81±3.74 mm and 29.99±3.91 mm respectively. The mean±SD neck length on posterior aspect of femur on right and left side was 35.90±4.07 mm and 35.97±4.17 mm respectively. The knowledge of variations in the parameters of dry femora will help the orthopedicians and also assist prosthetists to design a suitable prosthesis. These data also provide information about neck length of femur of Bangladeshi people and to compare these data to those from various other races thus it could contribute to demographic studies of fracture neck femur, osteoarthritis, hip joint ailments probability and management in Bangladeshi population.