Highly efficient enzymatic enrichment of n-3 polyunsaturated fatty acid glycerides via interfacial biocatalysis in Pickering emulsions

Liumei Wu; Dongming Li; Fuyang Bi; Chao Yu; Yufei Zhang; Mingming Zheng

doi:10.1016/j.foodchem.2024.142683

Highly efficient enzymatic enrichment of n-3 polyunsaturated fatty acid glycerides via interfacial biocatalysis in Pickering emulsions

Food Chem. 2024 Dec 28:470:142683. doi: 10.1016/j.foodchem.2024.142683. Online ahead of print.

Authors

Liumei Wu¹, Dongming Li², Fuyang Bi³, Chao Yu⁴, Yufei Zhang⁵, Mingming Zheng⁶

Affiliations

¹ Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Hubei Hongshan Laboratory, Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Wuhan 430062, China; College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
² College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
³ Xinjiang Uygur Autonomous Region Saihu Fishery Science and Technology Development Company Limited, Bortala Mongol Autonomous Prefecture, 833500, China.
⁴ CABIO Biotech (Wuhan) Co., Ltd. Wuhan East Lake High-tech Development Zone, Wuhan 430073, China.
⁵ Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Hubei Hongshan Laboratory, Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Wuhan 430062, China; Xinjiang Uygur Autonomous Region Saihu Fishery Science and Technology Development Company Limited, Bortala Mongol Autonomous Prefecture, 833500, China. Electronic address: zhangyufei@caas.cn.
⁶ Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Hubei Hongshan Laboratory, Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Wuhan 430062, China; Xinjiang Uygur Autonomous Region Saihu Fishery Science and Technology Development Company Limited, Bortala Mongol Autonomous Prefecture, 833500, China. Electronic address: zhengmingming@caas.cn.

PMID: 39742604
DOI: 10.1016/j.foodchem.2024.142683

Abstract

A novel Pickering interfacial biocatalysis (PIB) system has been, for the first time, successfully applied for the enzymatic selective hydrolysis of algae oils and fish oils to enrich n-3 PUFAs glycerides. Lipase AY 400SD was identified and adsorbed on hydrophobic hollow core-shell silica nanoparticles, resulting in the formation of the immobilized enzyme AY 400SD@HMSS-C₁₈. The biocatalyst was employed as an emulsifier to stabilize the water-in-oil Pickering emulsion, resulting in the successful construction of the PIB system. The newly developed PIB system resulted in a notable enhancement of the n-3 PUFAs content of the six oils, with a mean increase ranging from 9.17 % to 23.09 %, and with the recovery rate of n-3 PUFAs exceeded 90 %. The platform demonstrated to be stable and recyclable. The present research illustrated that the PIB has the potential to be a viable alternative for the purpose of enhancing the content of n-3 PUFAs in glycerides.

Keywords: Immobilized enzyme; Interfacial biocatalysis; Mesoporous silicon spheres; N-3 PUFAs enrichment; Pickering emulsion.