A 3D bioprinted potential colorectal tumor model based on decellularized matrix/gelatin methacryloyl/nanoclay/sodium alginate hydrogel

Xinyue Liu; Yan Shu; Jingjing Zhu; Huan Fang; Ya Su; Hailin Ma; Bing Li; Jie Xu; Yuen Yee Cheng; Bo Pan; Kedong Song

doi:10.1016/j.ijbiomac.2024.139346

A 3D bioprinted potential colorectal tumor model based on decellularized matrix/gelatin methacryloyl/nanoclay/sodium alginate hydrogel

Int J Biol Macromol. 2024 Dec 30:293:139346. doi: 10.1016/j.ijbiomac.2024.139346. Online ahead of print.

Authors

Xinyue Liu¹, Yan Shu¹, Jingjing Zhu¹, Huan Fang¹, Ya Su¹, Hailin Ma¹, Bing Li¹, Jie Xu², Yuen Yee Cheng³, Bo Pan⁴, Kedong Song⁵

Affiliations

¹ Cancer Hospital of Dalian University of Technology, State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
² Cancer Hospital of Dalian University of Technology, State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address: xuj@dlut.edu.cn.
³ Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology Sydney, NSW 2007, Australia. Electronic address: YuenYee.Cheng@uts.edu.au.
⁴ Department of Breast Surgery, The Second Hospital of Dalian Medical University, 467 Zhongshan Road, Shahekou District, Dalian, Liaoning 116023, China. Electronic address: bopan@dmu.edu.cn.
⁵ Cancer Hospital of Dalian University of Technology, State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address: Kedongsong@dlut.edu.cn.

PMID: 39743054
DOI: 10.1016/j.ijbiomac.2024.139346

Abstract

Colorectal cancer (CRC) is now the third most common cancer worldwide. However, the development cycle for anticancer drugs is lengthy and the failure rate is high, highlighting the urgent need for new tumor models for CRC-related research. The decellular matrix (dECM) offers numerous cell adhesion sites, proteoglycan and cytokines. Notably, porcine small intestine is rich in capillaries and lymphatic capillaries, which facilitates nutrient absorption. This study, we utilized dECM, along with methylacryloyl gelatin (GelMA), sodium alginate (SA) and nanoclay (NC) to create a hydrogel scaffold through 3D extrusion bioprinting. Human CRC cells (HCT8) were seeded onto the scaffold and their drug resistance was tested using 5-fluorouracil (5-FU). Our findings indicate that dECM enhances the hydrophilic properties, mechanical strength and biocompatibility of the scaffold. Furthermore, compared to traditional two-dimensional (2D) models, the three-dimensional (3D) scaffold supports the long-term growth of tumor spheres. After 2 days of 5-FU treatment, the cell survival rate reaches 88.06 ± 0.51 %. This suggests that our scaffold provides a promising alternative platform for in vitro research on cancer mechanisms, anti-cancer drug screening and new drug development.

Keywords: 3D extrusion bioprinting; 3D tumor model; Colorectal cancer; Decellularized matrix; Gelatin methacryloy.