Multimodal nanoenzyme-linked aptamer assay for Salmonella typhimurium based on catalysis and photothermal effect of PB@Au

Abstract

A composite nanomaterial of Prussian blue@gold nanoparticles (PB@Au) with catalytic and photothermal properties was proposed, which combined with anti-matrix interference aptamers to achieve robust specificity and sensitivity in the detection of Salmonella typhimurium (S. typhimurium). The detection probe, PB@Au-Aptamer (PB@Au-Apt), was designed to exhibit high specificity for the target and catalyze the signal generation to produce a color change, thereby enabling rapid detection. Additionally, the excellent photothermal performance of the PB@Au catalytic system was utilized for multimodal sensitive detection in the multimodal nanoenzyme-linked aptamer assay. Moreover, the utilization of both catalytic and photothermal dual-mode detection was mutually verified to enhance detection accuracy. Under optimal conditions, the detection of S. typhimurium in a sample can be completed in 2 h. The developed assay exhibited exceptional specificity in detecting S. typhimurium, with an impressive detection limit down to 23 CFU·mL^-1. Furthermore, the assay exhibited excellent repeatability and stability. Real sample analyses have proven the high reliability and practicality of this assay, highlighting its significant potential for applications in food safety testing.

Keywords: Salmonella typhimurium; Anti-matrix interference aptamers; Catalytic property; Microplate reader; Multimodal nanoenzyme-linked aptamer assay; Photothermal property; Prussian blue@gold nanoparticles; Visual color detection.