Inhibition of urate induced pyroptosis and reabsorption transporters overexpression in human renal tubular epithelial cells by low molecular weight fucoidan, as well as its anticoagulation and antioxidation

Jiayi Wu; Siwei Xiong; Zhiling Huang; Ping Luo; Huazhong Liu

doi:10.1016/j.ijbiomac.2025.139454

Inhibition of urate induced pyroptosis and reabsorption transporters overexpression in human renal tubular epithelial cells by low molecular weight fucoidan, as well as its anticoagulation and antioxidation

Int J Biol Macromol. 2025 Jan 2:139454. doi: 10.1016/j.ijbiomac.2025.139454. Online ahead of print.

Authors

Jiayi Wu¹, Siwei Xiong¹, Zhiling Huang¹, Ping Luo², Huazhong Liu³

Affiliations

¹ College of Chemistry and Environmental Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
² College of Chemistry and Environmental Sciences, Guangdong Ocean University, Zhanjiang 524088, China. Electronic address: luop@gdou.edu.cn.
³ College of Chemistry and Environmental Sciences, Guangdong Ocean University, Zhanjiang 524088, China. Electronic address: liuhz@gdou.edu.cn.

PMID: 39755308
DOI: 10.1016/j.ijbiomac.2025.139454

Abstract

Low molecular weight fucoidan (LMWF) has been proved to be more potent than its prototype, many degradation methods have been used to prepare LMWF. This study is conducted to further explore the biological activities of LMWF prepared by ultrasound based on anticoagulation, antioxidation, and inhibition of urate induced pyroptosis and reabsorption transporters overexpression in human renal tubular epithelial cells. Data revealed that ultrasound successfully degraded fucoidan to be LMWF, the product treated for no more than 2.5 h has stronger in vitro anticoagulant activity that reached the peak value at 2.5 h demonstrated by notably increased activated partial thromboplastin time (APTT) and prothrombin time (PT), but antioxidation progressively reduced along with treatment time indicated by decreased scavenging capacity of DPPH and hydroxyl radicals. LMWF with higher content of sulfate acid group possesses stronger inhibitory activity of urate-induced overexpression of urate-reabsorption transporters and pyroptosis in human renal tubular epithelial cells than native fucoidan. Degradation kinetics model of fucoidan exposed to ultrasonic treatment was best fitted to the second-order kinetic equation. These results indicate that LMWF is more effective for renal protection and anticoagulation than high molecular weight fucoidan, suggesting LMWF is a more promising marine drug.

Keywords: Biological activity; Low molecular weight fucoidan; Ultrasonication.