Electroactive membranes enhance in-situ alveolar ridge preservation via spatiotemporal electrical modulation of cell motility

Yanlan Wang; Shiqi Zhou; Xiaoshuang Wang; Dongheng Lu; Jinghong Yang; Yu Lu; Xiaolei Fan; Changhao Li; Yan Wang

doi:10.1016/j.biomaterials.2024.123077

Electroactive membranes enhance in-situ alveolar ridge preservation via spatiotemporal electrical modulation of cell motility

Biomaterials. 2024 Dec 31:317:123077. doi: 10.1016/j.biomaterials.2024.123077. Online ahead of print.

Authors

Yanlan Wang¹, Shiqi Zhou¹, Xiaoshuang Wang¹, Dongheng Lu¹, Jinghong Yang¹, Yu Lu¹, Xiaolei Fan¹, Changhao Li², Yan Wang³

Affiliations

¹ Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
² State Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Sanya, 572025, China. Electronic address: lichanghao@hainanu.edu.cn.
³ Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China. Electronic address: wangyan9@mail.sysu.edu.cn.

PMID: 39756273
DOI: 10.1016/j.biomaterials.2024.123077

Abstract

Post-extraction alveolar bone resorption invariably compromises implant placement and aesthetic restoration outcomes. Current non-resorbable membranes exhibit limited efficacy in alveolar ridge preservation (ARP) due to insufficient cell recruitment and osteoinductive capabilities. Herein, we introduce a multifunctional electroactive membrane (PPy-BTO/P(VDF-TrFE), PB/PT) designed to spatiotemporally regulate cell migration and osteogenesis, harmonizing with the socket healing process. Initially, the membrane's endogenous-level surface potential recruits stem cells from the socket. Subsequently, adherent cell-migration-triggered forces generate on-demand piezopotential, stimulating intracellular calcium ion fluctuations and activating the Ca²⁺/calcineurin/NFAT1 signaling pathway via Cav3.2 channels. This enhances cell motility and osteogenic differentiation predominantly in the coronal socket region, counteracting the natural healing trajectory. The membrane's self-powered energy supply, proportional to cell migration velocity and manifested as nanoparticle deformation, mitigates ridge shrinkage, both independently and in conjunction with bone grafts. This energy-autonomous membrane, based on the spatiotemporal modulation of cell motility, presents a novel approach for in-situ ARP treatment and the development of 4D bionic scaffolds.

Keywords: Alveolar ridge preservation; Bone regeneration; Cell-migration-mediated piezopotential; Electroactive membrane; Spatiotemporal electrical modulation.