Antiviral mechanism of Fuzhengjiedu San against porcine reproductive and respiratory syndrome virus

Xin-Qi Song; Xin-Yi Zhao; Wen-Shuang Chen; Li Yang; Dong-Yu Liu; Ya-Ping Chen

doi:10.1016/j.virol.2024.110382

Antiviral mechanism of Fuzhengjiedu San against porcine reproductive and respiratory syndrome virus

Virology. 2025 Jan 6:603:110382. doi: 10.1016/j.virol.2024.110382. Online ahead of print.

Authors

Xin-Qi Song¹, Xin-Yi Zhao¹, Wen-Shuang Chen¹, Li Yang¹, Dong-Yu Liu¹, Ya-Ping Chen²

Affiliations

¹ College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, PR China.
² College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, PR China. Electronic address: chenyaping0711@163.com.

PMID: 39798332
DOI: 10.1016/j.virol.2024.110382

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a viral infectious disease that can cause infection in pigs of different ages. The condition known as porcine reproductive and respiratory syndrome poses a serious risk to the world's pig business and results in significant financial losses. Fuzhengjiedu San (FZJDS) is a traditional Chinese medicine compound, the main components include:Radix Isatidis, Radix Astragali and Herba Epimedii. It has been widely used in clinical and experimental studies, showing a wide range of biological activity. However, it is not clear whether FZJDS has anti-PRRSV activity. We observed that FZJDS had significant antiviral activity in Marc-145 cells. And FZJDS could inhibit viral infection in the stages of viral internalization and replication. Furthermore, FZJDS can inhibit PRRSV replication by inhibiting the p53 signaling pathway to affect autophagy, and FZJDS can also inhibit PRRSV replication by inhibiting the PI3K/Akt pathway.We showed in this work that FZJDS inhibits PRRSV replication in vitro and offers a novel therapeutic approach for PRRSV infection.

Keywords: Antiviral; Fuzhengjiedu San(FZJDS); PI3K/Akt signaling pathway; Porcine reproductive and respiratory syndrome virus (PRRSV); p53 signaling pathway.